标题：A colorimetric, ratiometric and water-soluble fluorescent probe for simultaneously sensing glutathione and cysteine/homocysteine
作者：Dai, Xi; Wang, Zhao-Yang; Du, Zhi-Fang; Cui, Jie; Miao, Jun-Ying; Zhao, Bao-Xiang
作者机构：[Dai, Xi; Du, Zhi-Fang; Cui, Jie; Zhao, Bao-Xiang] Shandong Univ, Inst Organ Chem, Sch Chem & Chem Engn, Jinan 250100, Peoples R China.; [Wang, Zhao 更多
通讯作者地址：[Zhao, BX]Shandong Univ, Inst Organ Chem, Sch Chem & Chem Engn, Jinan 250100, Peoples R China.
来源：ANALYTICA CHIMICA ACTA
关键词：Ratiometric; Fluorescent probe; Glutathione; Coumarin; Cell imaging
摘要：A chlorinated coumarin-aldehyde was developed as a colorimetric and ratiometric fluorescent probe for distinguishing glutathione (GSH), cystenine (Cys) and homocysteine (Hcy). The GSH-induced substitution- cyclization and Cys/Hcy-induced substitution-rearrangement cascades lead to the corresponding thiol-coumarin-iminium cation and amino-coumarin-aldehyde with distinct photophysical properties. The probe can be used to simultaneously detect GSH and Cys/Hcy by visual determination based on distinct different colors - red and pale-yellow in PBS buffer solution by two reaction sites. From the linear relationship of fluorescence intensity and biothiols concentrations, it was determined that the limits of detection for GSH, Hcy and Cys are 0.08, 0.09 and 0.18 mu M, respectively. Furthermore, the probe was successfully used in living cell imaging with low cell toxicity. (C) 2015 Elsevier B.V. All rights reserved.