标题：Myrtol standardized affects mucociliary clearance
作者：Li, Ying Ying; Liu, Jing; Li, Chun Wei; Subramaniam, Somasundaram; Chao, Siew Shuen; Yu, Feng Gang; Cohen, Noam A.; Li, Shi; Wang, De 更多 作者机构：[Li, Ying Ying; Liu, Jing; Li, Chun Wei; Chao, Siew Shuen; Yu, Feng Gang; Wang, De Yun] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Otolaryngol, 1 更多
通讯作者地址：[Wang, DY]Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Otolaryngol, 1E Kent Ridge Rd, Singapore 119228, Singapore.
来源：INTERNATIONAL FORUM OF ALLERGY & RHINOLOGY
关键词：ciliogenesis; differentiation; human nasal epithelial stem/progenitor; cells; long-term treatment; mucus secretion; myrtol standardized;; short-term treatment
摘要：Background: Myrtol standardized (Gelomyrtol forte) has been shown to be effective in controlling nasal symptoms of rhinosinusitis by promoting mucociliary clearance. Our aim was to evaluate the short-and long-term effects of myrtol on ciliated columnar cells and goblet cells in an in-vitro setting.; Methods: Nasal epithelial cells were harvested (42 days) from an air-liquid interface (ALI) culture of human nasal epithelial stem/progenitor cells (hNESPCs), which was derived from biopsies of nasal inferior turbinate mucosa. Myrtol 0.1% was applied to the ALI culture system at 2 different time-points (day 0 and day 35) on progenitor and differentiated cells. Ciliary beat frequency (CBF), supernatant fluid, and ciliated and goblet cell markers were evaluated after short-(7 days) and long-term (42 days) treatment.; Results: In the long-term treatment with myrtol, there was an increase in cilia area (type IV beta-tubulin(+), 1.53-fold, p = 0.031) and ciliogenesis-related markers (Foxj1 and CP110) with no change in CBF, as compared with control. In addition, the short- term myrtol treatment group exhibited greater mucin secretion compared with control.; Conclusion: This study demonstrates, through cellular and molecular mechanisms, that myrtol standardized enhances the mucus production from goblet cells in the short term, and promotes ciliated cell differentiation in the long term. (C)2016 ARS-AAOA, LLC.