标题:Analytical strategies for detecting nanoparticle-protein interactions
作者:Li, L.;Mu, Q.;Zhang, B.;Yan, B.
作者机构:[Li, L] School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China;[ Mu, Q] School of Chemistry and Chemical Engineering, 更多
通讯作者:Yan, B
通讯作者地址:[Yan, B]Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Peoples R China.
来源:The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry
出版年:2010
卷:135
期:7
页码:1519-1530
DOI:10.1039/c0an00075b
摘要:A significant increase in biomedical applications of nanomaterials and their potential toxicity demands versatile analytical techniques to determine protein-nanoparticle (NP) interactions. These diverse analytical techniques are reviewed. Spectroscopic methods play a significant role in studying binding affinity, binding ratio, and binding mechanisms. To elucidate NP-proteome interactions, chromatography and electrophoresis techniques are applied to separate NP-bound proteins and matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to identify these proteins. Since NP-protein binding is a dynamic event, surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) are methods of choice to study the kinetics of NP-protein binding.
收录类别:SCOPUS;SCIE
WOS核心被引频次:53
Scopus被引频次:54
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-77953881040&doi=10.1039%2fc0an00075b&partnerID=40&md5=fbc2b9e322ade772626cac9e440005a5
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