标题：Modulate the structures and photophysical properties of pyrene-based far-red fluorescent cationic dyes by regio-effect
作者：Ding, Zhe; Tian, Minggang; Guo, Lifang; Liu, Zhi-qiang; Yu, Xiaoqiang
作者机构：[Ding, Zhe; Tian, Minggang; Guo, Lifang; Liu, Zhi-qiang; Yu, Xiaoqiang] Shandong Univ, State Key Lab Crystal Mat, Jinan 250100, Shandong, Peoples R Ch 更多
通讯作者：Liu, ZQ;Yu, XQ;Liu, Zhiqiang
通讯作者地址：[Liu, ZQ; Yu, XQ]Shandong Univ, State Key Lab Crystal Mat, Jinan 250100, Shandong, Peoples R China.
来源：SENSORS AND ACTUATORS B-CHEMICAL
关键词：Pyrene; Regioisomer; Far-red fluorescent dye; Fluorescent imaging
摘要：Two series of regioisomeric mono-substituted far-red fluorescent pyrene-indolium and pyrene-pyridinium conjugated dyes were synthesized, and their structures were ambiguously determined by single crystal X-ray diffractions. The photophysical properties of these pyrene-containing dyes in several solvents were investigated to figure out the modulation by changing the site of substituent on the pyrene moiety. The 4-substituted pyrene dyes Py-4 and PP-4 exceptionally demonstrated remarkable fluorescence quantum yield and much larger Stokes shift than their regioisomers (Delta(nu) over tilde of PP-4 is 7732 cm(-1) in acetonitrile). Preliminary cell staining and fluorescent imaging experiments indicated that t-Bu-Py-4 could be used as mitochondrial probe with good selectivity and low cytotoxicity.