标题：LIM Kinase 1 (LIMK1) interacts with Tropomyosin-related Kinase B (TrkB) and mediates Brain-derived Neurotrophic Factor (BDNF)-induced axonal elongation
作者机构：[Dong, Q] Dept. of Neurobiology, School of Medicine, Shandong University, No. 44 Wenhua Xi Rd., Jinan, Shandong 250012, China;[ Ji, Y.-S] Dept. of Neu 更多
通讯作者地址：[Chen, ZY]Shandong Univ, Dept Neurobiol, Sch Med, Shandong Prov Key Lab Mental Disorders, 44 Wenhua Xi Rd, Jinan 250012, Shandong, Peoples R China.
来源：The Journal of biological chemistry
摘要：BDNF/TrkB signaling plays critical roles in axonal outgrowth of neurons, the process of which requires the remodeling of the cytoskeleton structure, including microtubules and filamentous actin. However, the mechanism by which BDNF/TrkB signaling regulates cytoskeleton reorganization is still unclear. Here, we identified a novel interaction between LIMK1 and TrkB, which is required for the BDNF-induced axonal elongation. We demonstrated that BDNF-induced TrkB dimerization led to LIMK1 dimerization and transphosphorylation independent of TrkB kinase activity, which could further enhance the activation and stabilization of LIMK1. Moreover, activated LIMK1 translocated to the membrane fraction and phosphorylated its substrate cofilin, thus promoting actin polymerization and axonal elongation. Our findings provided evidence of a novel mechanism for the BDNF-mediated signal transduction leading to axonal elongation.