标题：Fluorimetric determinatin of nucleic acid using the enhancmenet of terbium-gadolinium-nucleic acid-cetylpyridine bromide system
作者：Lei Li;Jinghe Yang;Xia Wu;Changxia Sun;Guangjun Zhou
作者机构：[Li, L] Department of Chemistry, Laboratory of Colloid, Shandong University, Jinan 250100, China;[ Yang, J] Department of Chemistry, Laboratory of Col 更多
通讯作者地址：[Yang, JH]Shandong Univ, Dept Chem, Lab Colloid & Interface Chem Educ Minist, Jinan 250100, Peoples R China.
来源：Talanta: The International Journal of Pure and Applied Analytical Chemistry
关键词：nucleic acid;fluorescence intensity;cetylpyridine bromide
摘要：It is found that the fluoroesence intensity of Tb-cetylpyridine bromide (CPB)-nucleic acid system can be enhanced by La~(3+),Gd~(3+), Lu~(3+), Sc~(3+), and Y~3+), of which Gd~(3+) has the greatest enhancement. The experiments indicate that under the optimum condition, the fluorescence intensity of the system is in proportion to the concentration of nucleic acids in the range from 9 X 10~(-8) to 1 X 10~(-5) mol~(-1) for yeast RNA, from 1 X 10~(-7) to 1 X 10~(-5) g ml~(-1) for fish sperm DNA. The detection limits are 3.2 and 4.1 ng ml~(-1), respectively. This method has been used satisfactorily for the determination of both synthetic and actual samples. In comparison with most fluorescence method for the determination of nucleic acids, this method has higher sensitivity and stability.