标题:Danshensu protects vascular endothelia in a rat model of hyperhomocysteinemia
作者:Yang, Rui-xue; Huang, Shan-ying; Yan, Fang-fang; Lu, Xiao-ting; Xing, Yi-fan; Liu, Yan; Liu, Yun-fang; Zhao, Yu-xia
作者机构:[Liu, Yun-fang] Shandong Univ, Sch Med, Dept Diag, Jinan 250012, Peoples R China.; [Yang, Rui-xue; Huang, Shan-ying; Yan, Fang-fang; Lu, Xiao-ting; 更多
通讯作者:Liu, YF
通讯作者地址:[Liu, YF]Shandong Univ, Sch Med, Dept Diag, Jinan 250012, Peoples R China.
来源:ACTA PHARMACOLOGICA SINICA
出版年:2010
卷:31
期:10
页码:1395-1400
DOI:10.1038/aps.2010.167
关键词:hyperhomocysteinemia; danshensu; endothelin; nitric oxide; tumor; necrosis factor-alpha; intercellular adhesion molecule-1
摘要:Aim: To examine whether danshensu could protect vascular endothelia in a rat model of hyperhomocysteinemia.; Methods: The model was established by feeding rats with a methionine-rich diet (1 g.kg(-1)d(-1)) for 3 months. Immediately following the discontinuation of methionine-rich diet, rats were treated with danshensu (67.5 mg.kg(-1)d(-1), po) or saline for 3 additional months. One group of rats receiving vitamin mixture (folic acid, vitamin B12 and vitamin B6) was included as a positive control. One group of rats not exposed to methionine-rich diet was also included as a blank control. The expression of tumor necrosis factor-alpha (TNF-alpha) and intercellular adhesion molecule-1 (ICAM-1) protein in the descending aorta was examined using immunohistochemistry and Western blot. Homocysteine and blood concentration of endothelin and nitric oxide (NO) was also examined.; Results: Methionine-rich diet resulted in accumulation of "foam cells", up-regulated expression of TNF-alpha and ICAM-1 in the descending aorta, and significantly increased serum homocysteine. Plasma endothelin concentration was significantly increased; NO was decreased. Danshensu treatment, either simultaneous to methionine-rich diet or afterwards, attenuated the above mentioned changes.; Conclusion: Chronic treatment with danshensu could prevent/attenuate the formation of atherosclerosis. Potential mechanisms include inhibited expression of representative proinflammatory cytokines and adhesion molecules in arterial endothelia. Changes in homocysteine and circulating molecules that control vascular contraction/relaxation via endothelial cells (eg, endothelin and NO) were also implicated.
收录类别:SCOPUS;SCIE
WOS核心被引频次:28
Scopus被引频次:30
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-77957730552&doi=10.1038%2faps.2010.167&partnerID=40&md5=11fec2ebb24c9f338469963d6f8cfb6d
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