标题：WNT/beta-Catenin-Signaling Pathway Stimulates the Proliferation of Cultured Adult Human Sertoli Cells via Upregulation of C-myc Expression
作者：Li, Yi; Gao, Qing; Yin, Gang; Ding, Xiangyun; Hao, Jing
作者机构：[Li, Yi; Gao, Qing; Hao, Jing] Shandong Univ, Dept Histol & Embryol, Sch Med, Jinan 250012, Shandong, Peoples R China.; [Yin, Gang] Shandong Univ, Q 更多
通讯作者地址：[Hao, J]Shandong Univ, Dept Histol & Embryol, Sch Med, 44 Wenhuaxi Rd, Jinan 250012, Shandong, Peoples R China.
关键词：beta-catenin; sertoli cell; proliferation; c-myc; human
摘要：The role of WNT/beta-catenin-signaling pathway is critical in mouse Sertoli cell maturation and tumorigenesis. This study aims to examine the effects of WNT/beta-catenin signaling on the cultured adult human Sertoli cells and the underlying molecular mechanisms. Glycogen synthase kinase 3 beta (GSK-3 beta) inhibitors, SB216763 and lithium chloride (LiCl), were used to activate WNT/beta-catenin-signaling pathway. 5-Bromo-2'-deoxyuridine (BrdU) incorporation assay and flow cytometry were used to analyze the proliferation and cell cycle of cultured human Sertoli cells, respectively. C-myc expression was accessed by immunofluorescence, real-time polymerase chain reaction and Western blot. The effects of c-myc on Sertoli cell proliferation were investigated by RNA interference technology and BrdU incorporation assay. The results showed activation of WNT/beta-catenin signaling stimulated human Sertoli cell proliferation. Obvious increases in c-myc messenger RNA and protein expression were observed after SB216763 and LiCl treatments. Knockdown of c-myc expression attenuated the ability of WNT/beta-catenin signaling to stimulate the proliferation of human Sertoli cells. WNT/beta-catenin signaling enhances human Sertoli cell proliferation via upregulation of c-myc expression.