标题:WNT/beta-Catenin-Signaling Pathway Stimulates the Proliferation of Cultured Adult Human Sertoli Cells via Upregulation of C-myc Expression
作者:Li, Yi; Gao, Qing; Yin, Gang; Ding, Xiangyun; Hao, Jing
作者机构:[Li, Yi; Gao, Qing; Hao, Jing] Shandong Univ, Dept Histol & Embryol, Sch Med, Jinan 250012, Shandong, Peoples R China.; [Yin, Gang] Shandong Univ, Q 更多
通讯作者:Hao, J
通讯作者地址:[Hao, J]Shandong Univ, Dept Histol & Embryol, Sch Med, 44 Wenhuaxi Rd, Jinan 250012, Shandong, Peoples R China.
来源:REPRODUCTIVE SCIENCES
出版年:2012
卷:19
期:11
页码:1232-1240
DOI:10.1177/1933719112447126
关键词:beta-catenin; sertoli cell; proliferation; c-myc; human
摘要:The role of WNT/beta-catenin-signaling pathway is critical in mouse Sertoli cell maturation and tumorigenesis. This study aims to examine the effects of WNT/beta-catenin signaling on the cultured adult human Sertoli cells and the underlying molecular mechanisms. Glycogen synthase kinase 3 beta (GSK-3 beta) inhibitors, SB216763 and lithium chloride (LiCl), were used to activate WNT/beta-catenin-signaling pathway. 5-Bromo-2'-deoxyuridine (BrdU) incorporation assay and flow cytometry were used to analyze the proliferation and cell cycle of cultured human Sertoli cells, respectively. C-myc expression was accessed by immunofluorescence, real-time polymerase chain reaction and Western blot. The effects of c-myc on Sertoli cell proliferation were investigated by RNA interference technology and BrdU incorporation assay. The results showed activation of WNT/beta-catenin signaling stimulated human Sertoli cell proliferation. Obvious increases in c-myc messenger RNA and protein expression were observed after SB216763 and LiCl treatments. Knockdown of c-myc expression attenuated the ability of WNT/beta-catenin signaling to stimulate the proliferation of human Sertoli cells. WNT/beta-catenin signaling enhances human Sertoli cell proliferation via upregulation of c-myc expression.
收录类别:SCOPUS;SCIE
WOS核心被引频次:15
Scopus被引频次:17
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84867496933&doi=10.1177%2f1933719112447126&partnerID=40&md5=f6f4fe4c0ac43c4d48d1180ab2ddbd4e
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