标题：Induced production of steroids by co-cultivation of two endophytes from Mahonia fortunei
作者：Wang, Zi-Ru; Li, Gang; Ji, Li-Xia; Wang, Hang-Hang; Gao, Han; Peng, Xiao-Ping; Lou, Hong-Xiang
作者机构：[Wang, Zi-Ru; Li, Gang; Ji, Li-Xia; Wang, Hang-Hang; Gao, Han; Peng, Xiao-Ping; Lou, Hong-Xiang] Qingdao Univ, Sch Pharm, Dept Nat Med Chem & Pharmaco 更多
通讯作者地址：[Lou, HX]Shandong Univ, Sch Pharmaceut Sci, Dept Nat Prod Chem, Key Lab Chem Biol,Minist Educ, Jinan 250012, Shandong, Peoples R China.
关键词：Endophytes; Co-culture; Ergosterol derivatives; Antibacterial activity;; Cytotoxicity
摘要：A new ergosterol derivative, 23R-hydroxy-(20Z,24R)-ergosta-4,6,8(14),20(22)-tetraen-3-one (1), and a biosynthetically related known compound, (22E,24R)-ergosta-4,6,8(14),22-tetraen-3-one (2), were isolated from the co-culture between endophytic fungus Pleosporales sp. F46 and endophytic bacterium Bacillus wieclmannii Com1 both inhibiting in the medicinal plant Mahonia fortunei. The structure of the new compound 1 was determined by extensive spectroscopic analysis using HRMS and NMR, together with the modified Mosher's ester method. This is the first example of isolation of a ergosterol derivative with a Delta(20(22))-double bond in the side chain. Compound 1 exhibited moderate antibacterial efficacy against Staphylococcus aureus and no obvious cytotoxic activities against the cancer cell lines A549, MDA-MB-231 and Hct116. Our results not only reveal that compound 1 is a potent antibacterial lead compound, but also highlight the powder of co-cultivation for inducing the production of cryptic natural products from endophytes derived from the same host plant.