标题：Magnetic SERS Strip for Sensitive and Simultaneous Detection of Respiratory Viruses
作者：Wang, Chongwen; Wang, Chaoguang; Wang, Xiaolong; Wang, Keli; Zhu, Yanhui; Rong, Zhen; Wang, Weiyun; Xiao, Rui; Wang, Shengqi
作者机构：[Wang, Chongwen; Wang, Weiyun; Xiao, Rui; Wang, Shengqi] Anhui Agr Univ, Coll Life Sci, Hefei 230036, Anhui, Peoples R China.; [Wang, Chongwen; Wang 更多
通讯作者：Wang, Weiyun;Wang, WY;Xiao, R;Wang, SQ;Xiao, R;Wang, SQ;Wang, SQ
通讯作者地址：[Wang, WY; Xiao, R; Wang, SQ]Anhui Agr Univ, Coll Life Sci, Hefei 230036, Anhui, Peoples R China;[Xiao, R; Wang, SQ]Beijing Inst Radiat Med, Beijing K 更多
来源：ACS APPLIED MATERIALS & INTERFACES
关键词：lateral flow assay; magnetic SERS strip; Fe3O4@Ag; respiratory virus;; SERS
摘要：Rapid and early diagnosis of respiratory viruses is key to preventing infections from spreading and guiding treatments. Here, we developed a sensitive and quantitative surface-enhanced Raman scattering-based lateral flow immunoassay (SERS-based LFIA) strip for simultaneous detection of influenza A H1N1 virus and human adenovirus (HAdV) by using Fe3O4@Ag nanoparticles as magnetic SERS nanotags. The new type of Fe3O4@Ag magnetic tags, which were conjugated with dual-layer Raman dye molecules and target virus-capture antibodies, performs the following functions: specific recognition and magnetic enrichment of target viruses in the solution and SERS detection of the viruses on the strip. Based on this strategy, the magnetic SERS strip can directly be used for real biological samples without any sample pretreatment steps. The limits of detection for H1N1 and HAdV were 50 and 10 pfu/mL, respectively, which were 2000 times more sensitive than those from the standard colloidal gold strip method. Moreover, the proposed strip is easy to operate, rapid, stable, and can achieve high throughput and is thus a potential tool for early detection of virus infection.