标题：Omics Studies Revealed the Factors Involved in the Formation of Colony Boundary in Myxococcus xanthus
作者：Anwar, Mian Nabeel; Li, Zhi Feng; Gong, Ya; Singh, Raghvendra Pratap; Li, Yue-Zhong
作者机构：[Anwar, Mian Nabeel; Li, Zhi Feng; Gong, Ya; Singh, Raghvendra Pratap; Li, Yue-Zhong] Shandong Univ, Inst Microbial Technol, State Key Lab Microbial T 更多
通讯作者地址：[Li, YZ]Shandong Univ, Inst Microbial Technol, State Key Lab Microbial Technol, Qingdao 266237, Shandong, Peoples R China.
关键词：type VI secretion system; proteomics; Myxococcus xanthus; secondary; metabolites; microscopy; cold shock proteins
摘要：Two unrecognizable strains of the same bacterial species form a distinct colony boundary. During growth as colonies, Myxococcus xanthus uses multiple factors to establish cooperation between recognized strains and prevent interactions with unrecognized strains of the same species. Here, Delta MXAN_0049 is a mutant strain deficient in immunity for the paired nuclease gene, MXAN_0050, that has a function in the colony-merger incompatibility of Myxococcus xanthus DK1622. With the aim to investigate the factors involved in boundary formation, a proteome and metabolome study was employed. Visualization of the boundary between DK1622 and Delta MXAN_0049 was done scanning electron microscope (SEM), which displayed the presence of many damaged cells in the boundary. Proteome analysis of the DK1622- boundary disclosed many possible proteins, such as cold shock proteins, cell shape-determining protein MreC, along with a few pathways, such as RNA degradation, phenylalanine, tyrosine and tryptophan biosynthesis, and Type VI secretion system (T6SS), which may play major roles in the boundary formation. Metabolomics studies revealed various secondary metabolites that were significantly produced during boundary formation. Overall, the results concluded that multiple factors participated in the boundary formation in M. xanthus, leading to cellular damage that is helpful in solving the mystery of the boundary formation mechanism.