标题:Label electrochemical immunosensor for prostate-specific antigen based on graphene and silver hybridized mesoporous silica
作者:Li, Yueyun; Han, Jian; Chen, Runhai; Ren, Xiang; Wei, Qin
作者机构:[Li, Yueyun; Han, Jian; Chen, Runhai] Shandong Univ Technol, Sch Chem Engn, Zibo 255049, Peoples R China.; [Ren, Xiang; Wei, Qin] Univ Jinan, Shando 更多
通讯作者:Li, YY
通讯作者地址:[Li, YY]Shandong Univ Technol, Sch Chem Engn, Zibo 255049, Peoples R China.
来源:ANALYTICAL BIOCHEMISTRY
出版年:2015
卷:469
页码:76-82
DOI:10.1016/j.ab.2014.09.022
关键词:Immunosensor; Prostate-specific antigen; Silver hybridized mesoporous; silica; nanoparticles
摘要:Prostate-specific antigen (PSA), as the specificity of prostate cancer markers, has been widely used in prostate cancer diagnosis and screening. In this study, we fabricated an electrochemical immunosensor for PSA detection using the amino-functionalized graphene sheet-ferrocenecarboxaldehyde composite materials (NH2-GS@FCA) and silver hybridized mesoporous silica nanoparticles (Ag@NH2-MCM48). Under optimal conditions, the fabricated immunosensor showed a wide linear range with PSA concentration (0.01-10.0 ng.ml(-1)). Low detection limit (2 pg.ml(-1)) proved the high sensitivity. In addition, the immunosensor possessed good stability and reproducibility. Moreover, the application to PSA analysis in serum samples yielded satisfactory results. (C) 2014 Elsevier Inc. All rights reserved.
收录类别:SCIE
WOS核心被引频次:18
资源类型:期刊论文
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