标题：A new turn-on fluorescence probe for Zn2+in aqueous solution and imaging application in living cells
作者：Li, Meng-Meng ;Wang, Fang-Wu ;Wang, Xiao-Yun ;Zhang, Ting-Ting ;Xu, Yu ;Xiao, Yu ;Miao, Jun-Ying ;Zhao, Bao-Xiang
作者机构：[Li, Meng-Meng ;Zhang, Ting-Ting ;Xu, Yu ;Xiao, Yu ;Zhao, Bao-Xiang ] Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Sh 更多
来源：Analytica Chimica Acta
摘要：We designed and synthesized a new pyrazoline-based turn-on fluorescence probe for Zn2+by the reaction of chalcone and thiosemicarbazide. The structure of the probe was characterized by IR, NMR and HRMS spectroscopy. The probe (L) exhibits high selectivity and sensitivity for detecting Zn2+in buffered EtOH/HEPES solution (EtOH/HEPES=1/1, pH 7.2) with 80-fold fluorescence enhancement, which is superior to previous reports. Job's plot analysis revealed 1:1 stoichiometry between probe L and Zn2+ions. The association constant estimated by the Benesi-Hildebrand method and the detection limit were 3.92×103M-1and 5.2×10-7M, respectively. A proposed binding mode was confirmed by1H NMR titration experiments and density functional theory (DFT) calculations. The probe is cell-permeable and stable at the physiological pH range in biological systems. Because of its fast response to Zn2+, the probe can monitor Zn2+in living cells. Moreover, the selective binding of L and Zn2+was reversible with the addition of EDTA in buffered EtOH/HEPES solution and Zn2+could be imaged in SH-SY5Y neuron cells.
© 2014 Elsevier B.V.