标题：Oxidative damage induced by copper in mouse primary hepatocytes by single-cell analysis
作者：Jing, Mingyang; Liu, Yang; Song, Wei; Yan, Yunxing; Yan, Wenbao; Liu, Rutao
作者机构：[Jing, Mingyang; Liu, Yang; Song, Wei; Yan, Wenbao; Liu, Rutao] Shandong Univ, Sch Environm Sci & Engn, China Amer CRC Environm & Hlth, Jinan 250100, 更多
会议名称：2nd Contaminated Land, Ecological Assessment and Remediation (CLEAR 2014) Conference - Environmental Pollution and Remediation
会议日期：OCT 05-08, 2014
来源：ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
关键词：Copper; Oxidative damage; Mouse primary hepatocytes; Single-cell; analysis; Reactive oxygen species; Glutathione; DNA damage
摘要：Copper can disturb the intracellular redox balance, induce oxidative stress, and subsequently cause irreversible damage, leading to a variety of diseases. In the present study, mouse primary hepatocytes were chosen to elucidate the in vitro oxidative damage of short-term copper exposure (10-200 mu M) by single-cell analysis. We evaluated the toxicity of copper by reactive oxygen species (ROS), glutathione (GSH), and oxidative DNA damage at the single-cell level. Oxidative damage induced by copper was verified by the morphological changes, persistent elevations of excessive ROS and malondialdehyde (MDA), a decrease in GSH level, and the oxidative DNA damage. Furthermore, the average ROS generation, GSH consumption, and the indicators in DNA damage did not significantly change at relatively low concentrations (10 or 50 mu M), but we can find the alterations of parameters in some single cells clearly. Emphasis on the analysis of single cells is conducive to gain a better understanding on the toxicity of copper. This study will also complement studies on the environmental risk assessment of copper pollution.