标题：3D origami electrochemiluminescence immunodevice based on porous silver-paper electrode and nanoporous silver double-assisted signal amplification
作者：Li, Weiping; Li, Long; Li, Shuai; Wang, Xiu; Li, Meng; Wang, Shaowei; Yu, Jinghua
作者机构：[Li, Weiping; Li, Long; Li, Shuai; Wang, Xiu; Li, Meng; Yu, Jinghua] Univ Jinan, Sch Chem & Chem Engn, Shandong Univ, Key Lab Chem Sensing & Anal, Jin 更多
通讯作者地址：[Yu, JH]Univ Jinan, Sch Chem & Chem Engn, Shandong Univ, Key Lab Chem Sensing & Anal, Jinan 250022, Peoples R China.
来源：SENSORS AND ACTUATORS B-CHEMICAL
关键词：Electrochemiluminescence immunosensor; Tumor markers; Microfluidic; origami device; Point-of-care testing
摘要：A highly sensitive electrochemiluminescence (ECL) immunosensor combined with a 3D origami device for detection of carcinoembryonic antigen (CEA) was developed based on novel porous silver nanoparticles modified paper working electrode (Ag-PWE) as sensor platform and CdTe quantum dots functionalized nanoporous silver (QDs/NPS) as signal amplification label. The microfluidic origami device was fabricated by directly screen-printed electrodes on wax-patterned cellulose paper in bulk. The Ag-PWE was fabricated by a seed-mediated growth approach and served as an effective matrix for antibody attachment with good bioactivity, and stability. The ECL nanoprobe (Ab2RD5/NPS) was designed by covalent assembly of signal antibodies (Ab(2)) on QDs/NPS. After a sandwich immunoreaction, the functionalized NPS labels were captured onto the Ag-PWE surface. On the basis of the considerably amplified ECL signal and sandwich-type format, the proposed method successfully fulfilled the highly sensitive detection of CEA with a linear range of 0.5 pg mL(-1) to 20 ng mL(-1) with a detection limit of 0.12 pg mL(-1). This facile origami ECL immunodevice exhibited high sensitivity, specificity and excellent performance in real human serum assay, and could be applied in point-of-care testing of other tumor markers for remote regions and developing countries. (C) 2013 Elsevier B.V. All rights reserved.