标题：Bovine Serum Albumin Loaded Solid Lipid Nanoparticles Prepared by Double Emulsion Method
作者：Li Zhen; Li Xin-wei; Zheng Li-qiang; Lin Xiao-hong; Geng Fei; Yu Li
作者机构：[Li, Z] Key Laboratory of Colloid and Interface Chemistry, Ministry of Education, Shandong University, Jinan 250100, China;[ Li, X.-W] Key Laboratory 更多
通讯作者地址：[Zheng, LQ]Shandong Univ, Key Lab Colloid & Interface Chem, Minist Educ, Jinan 250100, Peoples R China.
关键词：Solid lipid nanoparticle;Double emulsion;Bovine serum albumin;Drug model
摘要：Solid lipid nanoparticles loaded with bovine serum albumin(BSA) were prepared by a double emulsion method.As the mass fraction of the model drug BSA increased from 0 to 15%,the particle size gradually increased.The physical stability of the nanoparticles was investigated by zeta potential measurement and they were shown to be quite stable.Fluorescence spectroscopy confirmed that the loaded position of BSA was on the interface between the inner aqueous phase and the solid lipid phase.Both Fourier-transform infrared spectroscopy and circular dichroism spectra indicate that BSA in the nanoparticles was not destroyed,but the secondary structure was disrupted slightly.