标题：Peanut genes encoding tetrapyrrole biosynthetic enzymes, AhHEMA1 and AhFC1, alleviating the salt stress in transgenic tobacco
作者：Yang, Sha; Zhao, Luying; Yan, Jianmei; Zhang, Jialei; Guo, Feng; Geng, Yun; Wang, Quan; Yang, Fangyuan; Wan, Shubo; Li, Xinguo
作者机构：[Yang, Sha; Zhao, Luying; Zhang, Jialei; Guo, Feng; Geng, Yun; Li, Xinguo] Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples 更多
通讯作者：Li, XG;Wan, SB
通讯作者地址：[Li, XG]Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China;[Wan, SB]Shandong Acad Agr Sci, Shandong Prov Key Lab Crop 更多
来源：PLANT PHYSIOLOGY AND BIOCHEMISTRY
关键词：Ferrochelatase; Glutamyl-tRNA reductase; Peanut (Arachis hypogaea L.);; Salt stress; Transgenic tobacco
摘要：Glutamyl-tRNA reductase1 (HMA1) and ferrochelatasel (FC1) are both expressed in response to salt stress in the biosynthetic pathway of tetrapyrroles. Peanut (Arachis hypogaea L.) HEMA1 and FC1 were isolated by RTPCR. The amino acid sequence encoded by the two genes showed high similarity with that in other plant species. The AhFC1 fusion protein was verified to function in chloroplast using Arabidopsis mesophyll protoplast. Sense and wild-type (WT) tobaccos were used to further study the physiological effects of AhHEMA1 and AhFC1. Compared with WT, the Herne contents and germination rate were higher in AhFC1 overexpressing plants under salt stress. Meanwhile, overexpressing AhHEMA1 also led to higher ALA and chlorophyll contents and multiple physiological changes under salt stress, such as higher activities of superoxide dismutase (SOD) and ascorbate peroxidase (APX), lower contents of reactive oxygen species (ROS) and slighter membrane damage. In addition, the activities of CAT, POD and APX in the AhFC1 overexpressing plants were significantly higher than that in WT lines under salt stress, but the activity of SOD between the WT plants and the transgenic plants did not exhibit significant differences. These results suggested that, peanut can enhance resistance to salt stress by improving the biosynthesis of tetrapyrrole biosynthetic.