标题：Arabidopsis thaliana in vitro shoot regeneration is impaired by silencing of TIR1
作者：Qiao, M.; Zhao, Z. -J.; Xiang, F. -N.
作者机构：[Qiao, M.; Zhao, Z. -J.; Xiang, F. -N.] Shandong Univ, Sch Life Sci, Key Lab Plant Cell Engn & Germplasm Innovat, Jinan 250100, Shandong, Peoples R Ch 更多
通讯作者地址：[Xiang, FN]Shandong Univ, Sch Life Sci, Key Lab Plant Cell Engn & Germplasm Innovat, Jinan 250100, Shandong, Peoples R China.
关键词：auxin receptor gene; auxin-rich medium; cytokinin related genes;; cytokinin-rich medium; root explants
摘要：Arabidopsis shoots regenerate from root explants through a two-step process consisting of pre-incubation on an auxin-rich callus induction medium (CIM), followed by transfer to a cytokinin-rich shoot induction medium (SIM). The auxin receptor gene TIR1 was up-regulated when explants were transferred to SIM. The CIM pre-incubation is required for its up-regulation. The tir1-1, TIR1 knockdown mutant, reduced the efficiency of shoot regeneration in tissue culture, while its over-expression mutant significantly improved efficiency. TIR1 promoter::GUS fusion analysis demonstrated that TIR1 expression was in the shoot and the newly emerging leaves. After 10 d on SIM, several cytokinin related genes (CDKB1;1, CKS1, IPT4 and ARR15), which associate with shoot regeneration, were up-regulated in plants over-expressing TIR1 and some of these were down-regulated in the tir1-1 mutant. Thus, TIR1 appears to be involved in regulating shoot regeneration.