标题:Sciatic nerve regeneration using a nerve growth factor-containing fibrin glue membrane
作者:Shengzhong Ma Changliang Peng Shiqing Wu Dongjin Wu Chunzheng Gao[1]
作者机构:[Ma Shengzhong] Department of Orthopedics, Second Affiliated Hospital of Shandong University, Jinan, Shandong 250033, China.;[Peng Changliang] Departm 更多
通讯作者地址:[Gao, CZ]Shandong Univ, Affiliated Hosp 2, Dept Orthoped, Jinan 250033, Shandong, Peoples R China.
来源:中国神经再生研究:英文版
出版年:2013
卷:8
期:36
DOI:10.3969/j.issn.1673-5374.2013.36.007
关键词:神经生长因子;周围神经再生;纤维蛋白胶;胶膜;P75NTR;雪旺氏细胞;神经营养因子;RT-PCR;
摘要:Our previous findings confirmed that the nerve growth factor-containing fibrin glue membrane provides a good microenvironment for peripheral nerve regeneration;however,the precise mechanism remains unclear.p75 neurotrophin receptor(p75NTR)plays an important role in the regulation of peripheral nerve regeneration.We hypothesized that a nerve growth factor-containing fibrin glue membrane can promote neural regeneration by up-regulating p75NTRexpression.In this study,we used a silicon nerve conduit to bridge a 15 mm-long sciatic nerve defect and injected a mixture of nerve growth factor and fibrin glue at the anastomotic site of the nerve conduit and the sciatic nerve.Through RT-PCR and western blot analysis,nerve growth factor-containing fibrin glue membrane significantly increased p75NTRmRNA and protein expression in the Schwann cells at the anastomotic site,in particular at 8 weeks after injection of the nerve growth factor/fibrin glue mixture.These results indicate that nerve growth factor-containing fibrin glue membrane can promote peripheral nerve regeneration by up-regulating p75NTRexpression in Schwann cells.
收录类别:CSCD;SCIE
WOS核心被引频次:5
资源类型:期刊论文
原文链接:http://lib.cqvip.com/qk/88507X/201336/48252534.html
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