标题:Repression of callus initiation by the miRNA-directed interaction of auxin-cytokinin in Arabidopsis thaliana
作者:Liu, Zhenhua; Li, Juan; Wang, Long; Li, Qiang; Lu, Qing; Yu, Yanchong; Li, Shuo; Bai, Ming-Yi; Hu, Yuxin; Xiang, Fengning
作者机构:[Liu, Zhenhua; Li, Juan; Wang, Long; Li, Qiang; Lu, Qing; Yu, Yanchong; Li, Shuo; Bai, Ming-Yi; Xiang, Fengning] Shandong Univ, Sch Life Sci, Key Lab 更多
通讯作者:Xiang, Fengning
通讯作者地址:[Xiang, FN]Shandong Univ, Sch Life Sci, Key Lab Plant Cell Engn & Germplasm Innovat, Jinan, Shandong, Peoples R China.
来源:PLANT JOURNAL
出版年:2016
卷:87
期:4
页码:391-402
DOI:10.1111/tpj.13211
关键词:miR160; ARF10; callus; auxin-cytokinin interaction; auxin; cytokinin;; Arabidopsis thaliana
摘要:In tissue culture systems plant cells can be induced to regenerate to whole plants. A particularly striking example of cellular reprogramming is seen in this regeneration process, which typically begins with the induction of an intermediate cell mass referred to as callus. The identity of the key genetic cues associated with callus formation is still largely unknown. Here a microRNA-directed phytohormonal interaction is described which represses callus initiation and formation in Arabidopsis thaliana. miR160 and ARF10 (At2g28350), a gene encoding an auxin response factor, were shown to exhibit a contrasting pattern of transcription during callus initiation from pericycle-like cells. The callus initiation is faster and more prolific in a miR160-resistant form of ARF10 (mARF10), but slower and less prolific in the transgenic line over-expressing miR160c (At5g46845), arf10 and arf10 arf16 mutants than that in the wild type. ARF10 repressed the expression of Arabidopsis Response Regulator15 (ARR15, At1g74890) via its direct binding to the gene's promoter. The loss of function of ARR15 enhanced callus initiation and partly rescued the phenotype induced by the transgene Pro35S:miR160c. Overexpression of ARR15 partly rescues the callus initiation defect of mARF10 plants. Our findings define miR160 as a key repressor of callus formation and reveal that the initiation of callus is repressed by miR160-directed interaction between auxin and cytokinin.
收录类别:EI;SCOPUS;SCIE
WOS核心被引频次:9
Scopus被引频次:10
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85027950535&doi=10.1111%2ftpj.13211&partnerID=40&md5=c87aef49c872c8135c07176eac15f707
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