标题：Long-Distance Control of Synapse Assembly by Target-Derived NGF
作者：Sharma, Nikhil; Deppmann, Christopher D.; Harrington, Anthony W.; Hillaire, Coryse St.; Chen, Zhe-Yu; Lee, Francis S.; Ginty, David D.
作者机构：[Sharma, Nikhil; Deppmann, Christopher D.; Harrington, Anthony W.; Hillaire, Coryse St.; Ginty, David D.] Johns Hopkins Univ, Sch Med, Solomon H Snyde 更多
通讯作者地址：[Ginty, DD]Johns Hopkins Univ, Sch Med, Solomon H Snyder Dept Neurosci, Baltimore, MD 21205 USA.
摘要：We report a role for long-distance retrograde neurotrophin signaling in the establishment of synapses in the sympathetic nervous system. Target-derived NGF is both necessary and sufficient for formation of postsynaptic specializations on dendrites of sympathetic neurons. This, in turn, is a prerequisite for formation of presynaptic specializations, but not preganglionic axonal ingrowth from the spinal cord into sympathetic ganglia. We also find that NGF-TrkA signaling endosomes travel from distal axons to cell bodies and dendrites where they promote PSD clustering. Furthermore, the p75 neurotrophin receptor restricts PSD formation, suggesting an important role for antagonistic NGF-TrkA and p75 signaling pathways during retrograde control of synapse establishment. Thus, in addition to defining the appropriate number of sympathetic neurons that survive the period of developmental cell death, target-derived NGF also exerts control over the degree of connectivity between the spinal cord and sympathetic ganglia through retrograde control of synapse assembly.