标题:Differentiation of lymphatic endothelial cells from bone marrow mesenchymal stem cells with VEGFS
作者:Wei,L.;Liu,Y.;Chen,G.;Fang,Y.;Song,X.;Dong,P.;Gao,J.;Liu,R.;Ding,Z.;Bi,Y.;Liu,Z.
作者机构:[Wei, L] Department of Anatomy, Shandong University School of Medicine, 44#, Wenhua Xi Road, Jinan, Shandong, 250012, China;[ Liu, Y] Provincial Key L 更多
通讯作者:Liu, ZY
通讯作者地址:[Liu, ZY]Shandong Univ, Sch Med, Dept Anat, 44 Wenhua Xi Rd, Jinan 250012, Shandong, Peoples R China.
来源:Lymphology
出版年:2012
卷:45
期:4
页码:177-187
关键词:Differentiation;Lymphatic endothelial cell;Mesenchymal stem cell (MSC);VEGF-A;VEGF-C
摘要:Although there have been many experimental studies demonstrating that bone marrow-derived mesenchymal stem cells (MSCs) have the potential to differentiate into mesenchymal tissues such as osteocytes, chondrocytes, and adipocytes in vivo and in vitro, little information is available regarding their potential to differentiate into lymphatic endothelial cells. Therefore, we chose to investigate differentiation of MSCs into lymphatic endothelial cells using stimulation with members of the vascular endothelial growth factor (VEGFs) family. Rat MSCs were isolated from bone marrow aspirate of Sprague-Dawley rats as previously described and characterized with flow cytometry for surface markers CD14, CD34, CD29, and CD90. Purified MSCs were plated and cultured in the presence of VEGF-A, VEGF-C, or the combination of both for 10 days. We examined the cells for Prox-1 and LYVE-1 by immunocytochemistry, RT-PCR, and Western blot analysis. Results demonstrated that compared to controls, cell differentiated with VEGF-A, VEGF-C and VEGF-A+VEGF-C expressed Prox-1 and LYVE-1. Our results indicate that MSCs induced by VEGFs are capable of differentiating into lymphatic endothelial-like cells in vitro, and this response has the potential to make them attractive candidates for the development of autologous tissue grafts for future therapy.
收录类别:SCOPUS;SCIE
WOS核心被引频次:3
Scopus被引频次:6
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84877089953&partnerID=40&md5=040d955434ad40cadb32a1d43f9bbf67
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