标题：The pH-sensing Rim101 pathway positively regulates the transcriptional expression of the calcium pump gene PMR1 to affect calcium sensitivity in budding yeast
作者：Yan H.; Fang T.; Xu H.; Jiang L.
作者机构：[Yan, H] Laboratory for Yeast Molecular and Cell Biology, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 更多
通讯作者地址：[Jiang, L] Laboratory for Yeast Molecular and Cell Biology, School of Agricultural Engineering and Food Science, Shandong University of TechnologyChin 更多
来源：Biochemical and Biophysical Research Communications
关键词：Calcium; Crz1; Nrg1; PMR1; Rim101; Saccharomyces cerevisiae
摘要：In Saccharomyces cerevisiae, the Rim101 pathway senses extracellular pH changes through a complex consisted of Rim8, Rim9 and Rim21 at the plasma membrane. Activation of this sensor complex induces a proteolytical complex composed of Rim13 and Rim20 and leads to the C-terminal processing and activation of the transcription factor Rim101. Deletion mutants for RIM8, RIM9, RIM13, RIM20, RIM21 and RIM101 causes yeast cells to be sensitive to calcium stress, but how they regulate calcium sensitivity remain unknown. Here we show that deletion mutations of these six Rim101 pathway components elevate the activation level of the calcium/calcineurin signaling and the transcriptional expression level of the vacuolar calcium pump gene PMC1, but lead to a reduction in transcriptional expression level of the ER/Golgi calcium pump gene PMR1 in yeast cells. Deletion of NRG1, encoding one of the repression targets of Rim101, rescues the transcriptional expression of PMR1 in all these mutants. Furthermore, ectopic expression of a constitutively active form of Rim101 or further deletion of NRG1 suppresses the calcium sensitivity of these six deletion mutants. Therefore, the pH-sensing Rim101 pathway positively regulates the transcriptional expression of PMR through its downstream target Nrg1 to affect the calcium sensitivity of yeast cells. © 2020 Elsevier Inc.