标题：Anti-tumor activity and the mechanism of SIP-S: A sulfated polysaccharide with anti-metastatic effect
作者：Zong, Aizhen; Liu, Yuhong; Zhang, Yan; Song, Xinlei; Shi, Yikang; Cao, Hongzhi; Liu, Chunhui; Cheng, Yanna; Jiang, Wenjie; Du, Fangl 更多 作者机构：[Zong, Aizhen; Zhang, Yan; Song, Xinlei; Liu, Chunhui; Jiang, Wenjie; Wang, Fengshan] Shandong Univ, Inst Biochem & Biotechnol Drugs, Sch Pharmaceut S 更多
通讯作者地址：[Wang, FS]Shandong Univ, Inst Biochem & Biotechnol Drugs, Sch Pharmaceut Sci, Key Lab Chem Biol,Minist Educ, 44 Wenhuaxi Rd, Jinan 250012, Shandong, P 更多
关键词：SIP-S; Cyclophosphamide; Anti-tumor activity; Immunoenhancing activity;; Apoptosis
摘要：Our previous studies demonstrated that SIP-S had anti-metastatic activity and inhibited the growth of metastatic foci. Here we report the anti-tumor and immunoregulatory potential of SIP-S.; SIP-S could significantly inhibit tumor growth in S180-bearing mice, and the inhibition rates was 43.7% at 30 mg/kg d. Besides, SIP-S could improve the thymus and spleen indices of S180-bearing mice and the mice treated with CTX. The combination of SIP-S (15 mg/kg d) with CTX (12.5 mg/kg d) showed higher anti-tumor potency than CTX (25 mg/kg d) alone. These results indicated that SIP-S had immunoenhancing and anticancer activity, and the immunoenhancing activity might be one mechanism for its anti-tumor activity.; Flow cytometry results showed that SIP-S could induce tumor cells apoptosis. Western blot analysis indicated that SIP-S could upregulate the expression of pro-apoptotic proteins, caspase-3, -8, -9 and Bax, and downregulate the expression of anti-apoptotic protein PARP-1 in tumor cells in a dose-dependent manner.; In summary, SIP-S has anti-tumor activity, which may be associated with its immunostimulating and pro-apoptotic activity. (C) 2015 Elsevier Ltd. All rights reserved.