标题：Improvement of cellulolytic enzyme production and performance by rational designing expression regulatory network and enzyme system composition
作者：Li, Zhonghai; Liu, Guodong; Qu, Yinbo
作者机构：[Li, Zhonghai; Liu, Guodong; Qu, Yinbo] Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Shandong, Peoples R China.; [Li, Zhonghai] Qi 更多
通讯作者地址：[Qu, YB]Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Shandong, Peoples R China.
关键词：Cellulase; Penicillium oxalicum; Transcriptional regulation; Rational; design
摘要：Filamentous fungi are considered as the most efficient producers expressing lignocellulose-degrading enzymes. Penicillium oxalicum strains possess extraordinary fungal lignocellulolytic enzyme systems and can efficiently utilize plant biomass. In recent years, the regulatory aspects of production of hydrolytic enzymes by P. oxalicum have been well established. This review aims to discuss the recent developments for the production of lignocellulolytic enzymes by P. oxalicum. The main cellulolytic transcription factors mediating the complex transcriptional-regulatory network are highlighted. The genome-wide identification of cellulolytic transcription factors, the cascade regulation network for cellulolytic gene expression, and the synergistic and dose-controlled regulation by cellulolytic regulators are discussed. A cellulase regulatory network sensitive to inducers in intracellular environments, the cross-talk of regulation of lignocellulose-degrading enzyme and amylase, and accessory enzymes are also demonstrated. Finally, strategies for the metabolic engineering of P. oxalicum, which show promising applications in the enzymatic hydrolysis for biochemical production, are established. (C) 2017 Published by Elsevier Ltd.