标题：Long non-coding RNA-CCAT2 promotes the occurrence of non-small cell lung cancer by regulating the Wnt/-catenin signaling pathway
作者：Zhao, Chengling; Qiao, Chenchen; Zong, Liguo; Chen, Yuqing
作者机构：[Zhao, Chengling] Shandong Univ, Sch Med, Sch Med, Jinan 250100, Shandong, Peoples R China.; [Zhao, Chengling; Chen, Yuqing] Bengbu Med Coll, Affili 更多
通讯作者地址：[Zhao, CL]Bengbu Med Coll, Affiliated Hosp 1, Dept Respirat, Anhui Clin & Preclin Key Lab Resp Dis, 803 Changhuai Rd, Bengbu 233004, Anhui, Peoples R 更多
关键词：non-small cell lung carcinoma; colon cancer-associated transcript 2;; Wnt; -catenin signaling pathway
摘要：The present study aimed to investigate the biological function of colon cancer-associated transcript 2 (CCAT2) in the occurrence and progression of non-small cell lung carcinoma (NSCLC) and its potential use in the early diagnosis and molecular-targeted therapy of NSCLC. The tumor tissues, para-carcinoma tissues and associated clinical data of 36 patients with NSCLC were collected in order to detect the expression of CCAT2 and assess the impact of factors including histopathological type, Tumor-Node-Metastasis stage and lymph node metastasis on CCAT2 expression. The lung cancer NCI-H1975 cell line was transfected with a small interfering RNA (siRNA) plasmid to determine the effect of si-CCAT2 on NSCLC proliferation, invasion and metastasis. The effect of si-CCAT2 on the expression of nuclear and cytoplasmic -catenin protein in the lung cancer NCI-H1975 cell line was detected using western blot analysis. The expression levels of CCAT2 in the tumor tissues of patients with NSCLC were significantly higher than those in the normal para-carcinoma tissues (t=8.580, P<0.01). Subsequent to CCAT2 silencing, the proliferation and invasive abilities of NCI-H1975 cells were significantly decreased compared with control cells (P<0.05). In the si-CCAT2 group, the level of nuclear and cytoplasmic -catenin proteins was decreased, and the activity of the Wnt signaling pathway was significantly inhibited compared with the control cells (P<0.01), and a synergistic effect was exerted with the Wnt signaling inhibitor FH535. CCAT2 may therefore promote the occurrence of NSCLC by regulating the Wnt/-catenin signaling pathway.