标题:Immunomodulatory effects of xanthan gum in LPS-stimulated RAW 264.7 macrophages
作者:Liu, Fuyan; Zhang, Xiaofeng; Ling, Peixue; Liao, Joshua; Zhao, Mingsheng; Mei, Li; Shao, Huarong; Jiang, Peng; Song, Zhigang; Chen, 更多
作者机构:[Liu, Fuyan; Ling, Peixue; Mei, Li; Shao, Huarong; Song, Zhigang; Chen, Qixin; Wang, Fengshan] Shandong Univ, Sch Pharmaceut Sci, Jinan 250012, People 更多
通讯作者:Ling, Peixue
通讯作者地址:[Wang, FS]Shandong Univ, Sch Pharmaceut Sci, Jinan 250012, Peoples R China;[Ling, PX]Shandong Acad Pharmaceut Sci, Jinan 250101, Peoples R China.
来源:CARBOHYDRATE POLYMERS
出版年:2017
卷:169
页码:65-74
DOI:10.1016/j.carbpol.2017.04.003
关键词:Xanthan gum; RAW264.7macrophages; Immunomodulatory; SPR analysis; TLR4;; Anti-inflammatory
摘要:In this study, we evaluated the immunomodulatory effects of xanthan gum (XG) in RAW264.7 macrophages and the underlying molecular mechanisms. We used scanning electron microscopy (SEM) to analyze the morphology of XG-treated RAW264.7 cells with and without lipopolysaccharide (LPS) stimulation and investigated the subsequent effects on nitric oxide (NO), interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), tumor necrosis factor (INF-alpha), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX 2) levels in LPS-activated mouse RAW264.7 macrophages. We also analyzed the binding affinity of XG to Toll-like receptor 4 (TLR4) with surface plasmon resonance (SPR) analysis and observed that XG decreased NO, IL-6 and INF-alpha secretion into the culture medium and iNOS and COX-2 protein levels induced by LPS. This study reveals a two-way immunomodulatory effect of XG on inflammatory mediators in RAW264.7 macrophages that may involve the TLR4 signal pathway, providing a pharmacological basis for the use of XG in the control of inflammatory disorders. (C) 2017 Published by Elsevier Ltd.
收录类别:EI;SCOPUS;SCIE
WOS核心被引频次:3
Scopus被引频次:5
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85017221705&doi=10.1016%2fj.carbpol.2017.04.003&partnerID=40&md5=93cfe9cf413becfd735de11399341d77
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