标题：Gene Expression Profiles in Chemokine (C-C Motif) Ligand 21-Overexpressing Pancreatic Cancer Cells
作者：Cui K.; Zou H.; Shi M.; Ou Y.; Han L.; Zhang B.; Hu D.; Li S.
作者机构：[Cui, K] Shandong Cancer Hospital affiliated to Shandong University, Jinan, Shandong 250117, China, Shandong Academy of Medical Sciences, Jinan, Shan 更多
通讯作者地址：[Hu, D] Shandong Academy of Medical SciencesChina;
来源：Pathology and Oncology Research
关键词：Chemokine (C-C motif) ligand 21; DNA microarray; Lentiviral transduction; Matrix metallopeptidase-9; Pancreatic cancer cell
摘要：Chemokine (C-C Motif) ligand 21 (CCL21) plays an important role in tumor immunity. However, the molecular mechanisms by which CCL21 regulates tumor immunity remain largely unknown. In this study, we successfully generated a lentiviral vector expressing human CCL21 (Lenti-hCCL21), which was confirmed by biological assays. The Lenti-hCCL21 was transduced into PANC-1 cells, a chemokine (C-C motif) receptor 7 (CCR7)-positive human pancreatic cancer cell line. We used the scratch wound and transwell assays to measure cell migration of the CCL21-overexpressing PANC-1 cells. A DNA microarray assay was performed to determine gene expression profiles. The results showed that CCL21 lentiviral transduction significantly up- or down-regulated a panel of tumor-associated genes, although CCL21 appeared to have no effect on PANC-1 cell migration. Importantly, CCL21 promoted matrix metallopeptidase-9 (MMP-9) expression in PANC-1 cells. CCL21 regulates pancreatic cancer immunity possibly through governing the expression of a panel of tumor-associated genes, including MMP-9. © 2018 The Author(s)