标题：From inflammatory reactions to neurotransmitter changes: Implications for understanding the neurobehavioral changes in mice chronically infected with Toxoplasma gondii
作者：Wang, Ting; Sun, Xiahui; Qin, Wen; Zhang, Xiaoli; Wu, Leilei; Li, Yan; Zhou, Chunxue; Zhou, Huaiyu; He, Shenyi; Cong, Hua
作者机构：[Wang, Ting; Sun, Xiahui; Wu, Leilei; Li, Yan; Zhou, Chunxue; Zhou, Huaiyu; He, Shenyi; Cong, Hua] Shandong Univ, Sch Med, Dept Human Parasitol, Jinan 更多
通讯作者地址：[Cong, H]Shandong Univ, Sch Med, Dept Human Parasitol, Jinan, Shandong, Peoples R China.
来源：BEHAVIOURAL BRAIN RESEARCH
关键词：Toxoplasma gondii; Neurobehavior; Neuroinflammation; NF-kappa B pathway;; Neurotransmitters
摘要：Toxoplasma gondii is a protozoan parasite that can cause a latent infection in the central nervous system, leading to neurobehavioral abnormalities in the host. However, the mechanism underlying these changes remains relatively unexplored.; In this study, we detected behavioral changes, pathological injury, secretion of neurotransmitters and related signal pathway in mice infected by T. gondii using behavioral test, histopathology, immunofluorescence staining, western blotting, HPLC and real time PCR. Mice showed neurobehavioral disturbances two months after infection with T. gondii. Histopathology revealed the activation of astrocytes and microglia, apoptosis of neurons and decreases in synapses in the brain of infected mice. Excessive secretion of cytokines and chemokines was detected in the brains of mice infected by T. gondii compared to uninfected mice. Furthermore, T. gondii infection led to abnormalities in neurotransmitters and the activation of NF-kappa B and dopamine (DA) signaling pathways in the infected mice. In conclusion, excessive activation of the inflammation in the brain could induce neuronal apoptosis in mice chronically infected with T. gondii. Dysregulation of the dopaminergic neurotransmitter could provide an explanation of neurobehavioral disorders in infected hosts.