标题：Dual crosslinked chondroitin sulfate injectable hydrogel formed via continuous Diels-Alder (DA) click chemistry for bone repair
作者：Bai, Xiao; Lu, Shaoyu; Cao, Zhen; Ni, Boli; Wang, Xin; Ning, Piao; Ma, Dongyang; Wei, Hua; Liu, Mingzhu
作者机构：[Bai, Xiao; Lu, Shaoyu; Ning, Piao; Wei, Hua; Liu, Mingzhu] Lanzhou Univ, State Key Lab Appl Organ Chem, Key Lab Nonferrous Met Chem & Resources Utili 更多
通讯作者地址：[Lu, SY; Liu, MZ]Lanzhou Univ, State Key Lab Appl Organ Chem, Key Lab Nonferrous Met Chem & Resources Utilizat, Lanzhou 730000, Peoples R China;[Lu, S 更多
关键词：Diels-Alder; Dual crosslinked; Injectable hydrogel; Cranial bone tissue; engineering; Scaffold
摘要：In the present work, a thermosensetive copolymer with a low gelation concentration under 37 degrees C, F127@ChS (F127 crosslinked chondroitin sulfate) was synthesized via DA click chemistry between F127-AMI (maleimido terminated F127) and ChS-furan (furfurylamine grafted chondroitin sulfate). Then, dual crosslinked hydrogels were prepared based on F127@ChS and PEG-AMI (maleimido terminated polyethylene glycol). The physical crosslinking of F127@ChS affords the hydrogel fast gelation behavior, while in situ DA click reaction occurred between F127@ChS and PEG-AMI affords the hydrogel system covalent crosslinking. The dual crosslinked injectable hydrogel was applied as scaffold to load BMP-4 for rat cranial defect repair. As indicated by X-ray imaging, cranial digital images and histological (HE and Masson) staining analysis, new bone tissues were formed in the defected area after 12 weeks repair. The results demonstrate that the novel dual crosslinked injectable hydrogel offer an interesting option for cranial bone tissue engineering. (C) 2017 Elsevier Ltd. All rights reserved.