标题： [Simultaneous determination of four active compounds in tiangou capsules by HPLC].
作者： Wang, Xin; Li, Lingjun; Dai, Long
作者机构：[ Wang, Xin; Wang X] Shandong University of Traditional Chinese Medicine, Jinan 250355, China. email@example.com;[ Li, Lingjun; Li L];[ Dai, Lo 更多
来源： Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
摘要： OBJECTIVE: To establish a method for quantitative analysis of gastrodin, baicalin, paeonolum and pinoresinol diglucoside in Tiangou capsules by HPLC. METHOD: The separation was performed on SinoChrom ODS-BP column (4.6 mm x 150 mm, 5 microm). The mobile phase consisted of acetonitrile and 0.2% phosphoric acid aqueous solution with gradient elution program. The flow rate was 1.0 ml x min(-1) and the column temperature was 30 degrees C. UV detection wavelength was set at 230 nm. RESULT: The linear ranges of gastrodin, baicalin, paeonolum and pinoresinol diglucoside were 0.1168-1.1680, 0.1142-1.1420, 0.0512-0.5120, 0.0556-0.5560 microg, respectively. The average recoveries of gastrodin, baicalin, paeonolum and pinoresinol diglucoside were 100.3% (RSD 0.53%), 99.96% (RSD 1.15%), 99.90% (RSD 1.17%), 99.97% (RSD 1.62%), respectively. CONCLUSION: The method is accurate, simple, feasible and reliable, and is available for the quality control of Tiangou capsules.