标题：Apoptosis induced by low-concentration ethanol in hepatocellular carcinoma cell strains and down-regulated AFP and survivin analysis by proteomic technology
作者：Kai, Xin ;Li, Juan ;Huang, Sexin ;Bai, Zengliang
作者机构：[Kai, Xin ;Li, Juan ;Huang, Sexin ;Bai, Zengliang ] Laboratory of Developmental Immunology, College of Life Science, Shandong University, Jinan, 25010 更多
来源：World Academy of Science, Engineering and Technology
关键词：Ethanol; Hepatocellular carcinoma; Proteomics; Survivin and AFP
摘要：Ethanol is generally used as a therapeutic reagent against Hepatocellular carcinoma (HCC or hepatoma) worldwide, as it can induce Hepatocellular carcinoma cell apoptosis at low concentration through a multifactorial process regulated by several unknown proteins. This paper provides a simple and available proteomic strategy for exploring differentially expressed proteins in the apoptotic pathway. The appropriate concentrations of ethanol required to induce HepG2 cell apoptosis were first assessed by MTT assay, Gisma and fluorescence staining. Next, the central proteins involved in the apoptosis pathway processs were determined using 2D-PAGE, SDS-PAGE, and bio-software analysis. Finally the downregulation of two proteins, AFP and survivin, were determined by immunocytochemistry and reverse transcriptase PCR (RT-PCR) technology. The simple, useful method demonstrated here provides a new approach to proteomic analysis in key bio-regulating process including proliferation, differentiation, apoptosis, immunity and metastasis.