标题:RETRACTED: The role of cystatin C in vascular remodeling of balloon-injured abdominal aorta of rabbits (Retracted article. See vol. 42, pg. 1475, 2015)
作者:Wu, Xiang-Jun; Dong, Zhao-Qiang; Lu, Qing-Hua
作者机构:[Wu, Xiang-Jun; Dong, Zhao-Qiang; Lu, Qing-Hua] Shandong Univ, Hosp 2, Dept Cardiovasc Med, Jinan 250033, Peoples R China.; [Wu, Xiang-Jun] Binzhou 更多
通讯作者:Lu, QH
通讯作者地址:[Lu, QH]Shandong Univ, Hosp 2, Dept Cardiovasc Med, Beiyuan St 247, Jinan 250033, Peoples R China.
来源:MOLECULAR BIOLOGY REPORTS
出版年:2014
卷:41
期:9
页码:6225-6231
DOI:10.1007/s11033-014-3502-1
关键词:Cystatin C; Balloon dilatation; Abdominal aorta; Vascular remodeling
摘要:This study aimed to evaluate the role of cystatin C (CysC) in the vascular remodeling of balloon-injured abdominal aorta of rabbits. Forty-eight New Zealand white rabbits were randomly divided into three groups: the balloon-injured injury group (n = 16), the CysC monoclonal antibody group (n = 16), and the sham-operative group (n = 16). Serum CysC levels were detected by enzyme linked immunosorbent assay. Changes in adventitial area, adventitial thickness, lumen area (LA), neointimal area (IA), internal elastic lamina area (IELA), external elastic lamina area (EELA), vascular remodeling index (VRI) and residual stenosis (RS) were measured by the Leica image analysis system. Immunohistochemical analysis of alpha-smooth muscle actin (alpha-SMA) and proliferating cell nuclear antigen (PCNA) were performed. Serum CysC levels of rabbits in the balloon-injured injury group were significantly higher than those in the CysC monoclonal antibody group and the sham-operative group (both P < 0.05). At 6 weeks after balloon injury, the adventitial area and thickness, LA, IA, IELA and EELA in the balloon-injured injury group were also higher than those in the CysC monoclonal antibody and sham-operative groups (all P < 0.05). In addition, the balloon-injured injury group showed higher VRI and RS than those of the CysC monoclonal antibody group (both P < 0.05). The positive expression of alpha-SMA in the vascular adventitia and media in the balloon-injured group were higher than that of the CysC monoclonal antibody and sham-operative groups. The balloon-injured group also showed a stronger expression of alpha-SMA in the neointima than that of the CysC monoclonal antibody group. There was a strong positive expression of PCNA in the vascular adventitia and neointima in the balloon-injured and CysC monoclonal antibody groups. However, the number of PCNA-positive cells in the balloon-injured group was higher than that of the CysC monoclonal antibody group (25.45 +/- A 4.21 vs. 6.75 +/- A 1.11, P = 0.003). Our findings provide empirical evidence that serum CysC levels may play an important role in the vascular remodeling of balloon-injured abdominal aorta of rabbits.
收录类别:SCOPUS;SCIE
WOS核心被引频次:2
Scopus被引频次:1
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84906935025&doi=10.1007%2fs11033-014-3502-1&partnerID=40&md5=2b125b1290a9899bf471781c025fce23
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