标题：Life-long in vivo cell-lineage tracing shows that no oogenesis originates from putative germline stem cells in adult mice
作者：Zhang, Hua; Liu, Lian; Li, Xin; Busayavalasa, Kiran; Shen, Yan; Hovatta, Outi; Gustafsson, Jan-Ake; Liu, Kui
作者机构：[Zhang, Hua; Li, Xin; Busayavalasa, Kiran; Shen, Yan; Liu, Kui] Univ Gothenburg, Dept Chem & Mol Biol, SE-40530 Gothenburg, Sweden.; [Liu, Lian] Sha 更多
通讯作者地址：[Gustafsson, JA]Univ Houston, Ctr Nucl Receptors & Cell Signaling, Houston, TX 77204 USA.
来源：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
关键词：genetically modified mouse models; oocyte tracing; follicle tracing;; life-long observations; no postnatal oocyte regeneration
摘要：Whether or not oocyte regeneration occurs in adult life has been the subject of much debate. In this study, we have traced germ-cell lineages over the life spans of three genetically modified mouse models and provide direct evidence that oogenesis does not originate from any germline stem cells (GSCs) in adult mice. By selective ablation of all existing oocytes in a Gdf9-Cre;iDTR mouse model, we have demonstrated that no new germ cells were ever regenerated under pathological conditions. By in vivo tracing of oocytes and follicles in the Sohlh1-CreER(T2);R26R and Foxl2-CreER(T2);mT/mG mouse models, respectively, we have shown that the initial pool of oocytes is the only source of germ cells throughout the life span of the mice and that no adult oogenesis ever occurs under physiological conditions. Our findings clearly show that there are no GSCs that contribute to adult oogenesis in mice and that the initial pool of oocytes formed in early life is the only source of germ cells throughout the entire reproductive life span.