标题：miR-151-5p modulates APH1a expression to participate in contextual fear memory formation
作者：Xu, Xu-Feng; Wang, You-Cui; Zong, Liang; Wang, Xiao-Long
作者机构：[Xu, Xu-Feng; Wang, You-Cui] Qingdao Univ, Sch Basic Med, Inst Brain Sci & Dis, Qingdao 266001, Shandong, Peoples R China.; [Xu, Xu-Feng] Univ Ottaw 更多
通讯作者：Xu, XF;Wang, XL
通讯作者地址：[Xu, XF]Qingdao Univ, Sch Basic Med, Inst Brain Sci & Dis, Qingdao 266001, Shandong, Peoples R China;[Wang, XL]Shandong Univ, Qilu Hosp, Dept Breast S 更多
关键词：mir-151-5p; memory; APH1a; hippocampus
摘要：Long-term memory formation requires gene expression and new protein synthesis. MicroRNAs (miRNAs), a family of small non-coding RNAs that inhibit target gene mRNA expression, are involved in new memory formation. In this study, elevated miR-151-5p (miR-151) levels were found to be responsible for hippocampal contextual fear memory formation. Using a luciferase reporter assay, we demonstrated that miR-151 targets APH1a, a protein that has been identified as a key factor in -secretase activity, namely APH1a. Blocking miR-151 can upregulate APH1a protein levels and subsequently impair hippocampal fear memory formation. These results indicate that miR-151 is involved in hippocampal contextual fear memory by inhibiting APH1a protein expression. This work provides novel evidence for the role of miRNAs in memory formation and demonstrates the implication of APH1a protein in miRNA processing in the adult brain.