标题:In Vitro Reconstitution of a PKS Pathway for the Biosynthesis of Galbonolides in Streptomyces sp LZ35
作者:Liu, Chao; Zhu, Jing; Li, Yaoyao; Zhang, Juanli; Lu, Chunhua; Wang, Haoxin; Shen, Yuemao
作者机构:[Liu, Chao; Zhu, Jing; Wang, Haoxin; Shen, Yuemao] Shandong Univ, State Key Lab Microbial Technol, Sch Life Sci, Jinan 250100, Shandong, Peoples R Chi 更多
通讯作者:Wang, HX
通讯作者地址:[Wang, HX]Shandong Univ, State Key Lab Microbial Technol, Sch Life Sci, 27 Shanda South Rd, Jinan 250100, Shandong, Peoples R China.
来源:CHEMBIOCHEM
出版年:2015
卷:16
期:6
页码:998-1007
DOI:10.1002/cbic.201500017
关键词:biosynthesis; galbonolides; hybrid pathway; macrolides; polyketides
摘要:The galbonolides are 14-membered macrolide antibiotics with a macrocyclic backbone similar to that of erythromycins. Galbonolides exhibit broad-spectrum antifungal activities. Retro-biosynthetic analysis suggests that the backbone of galbonolides is assembled by a type I modular polyketide synthase (PKS). Unexpectedly, the galbonolide biosynthetic gene cluster, gbn, in Streptomyces sp. LZ35 encodes a hybrid fatty acid synthase (FAS)-PKS pathway. In vitro reconstitution revealed the functions of GbnA (an AT-ACP didomain protein), GbnC (a FabH-like enzyme), and GbnB (a novel multidomain PKS module without AT and ACP domains) responsible for assembling the backbone of galbonolides, respectively. To our knowledge, this study is the first biochemical characterization of a hybrid FAS-PKS pathway for the biosynthesis of 14-membered macrolides. The identification of this pathway provides insights into the evolution of PKSs and could facilitate the design of modular pools for synthetic biology.
收录类别:SCOPUS;SCIE
WOS核心被引频次:4
Scopus被引频次:6
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84926385210&doi=10.1002%2fcbic.201500017&partnerID=40&md5=0f0545944e31bad9de61b7f4b7fa4477
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