标题：Determination of fucoidan in rat plasma by HPLC and its application in pharmacokinetics
作者：Zhang, E.; Chu, Fulong; Zhao, Tongde; Chai, Yuchao; Liang, Hao; Song, Shuliang; Ji, Aiguo
作者机构：[Zhang, E.; Chu, Fulong; Zhao, Tongde; Chai, Yuchao; Liang, Hao; Song, Shuliang; Ji, Aiguo] Shandong Univ, Marine Coll, Weihai, Peoples R China.; [Z 更多
通讯作者：Ji, AG;Ji, AG;Ji, AG
通讯作者地址：[Ji, AG]Shandong Univ, Marine Coll, Weihai, Peoples R China;[Ji, AG]Shandong Univ, Weihai Int Biotechnol Res & Dev Ctr, Weihai, Peoples R China;[Ji, A 更多
来源：PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES
关键词：Fucoidan; FITC labelling; Laminaria japonica; HPLC with fluorescence; detection; validation
摘要：This is a new expanded method of determining the characterisation of fucoidan from Laminaria japonica (kelp) in rat plasma by high-performance liquid chromatography (HPLC) with fluorescence detection. We tagged fucoidan by fluoresce in isothiocyanate (FITC) for tracking and treated the plasma samples via protein precipitation with 10% trichloroacetic acid and methanol. Column chromatography separation was on a TSK-G4000sw column (7.8 mm x 300 mm, 5 nm) by elution with 0.15 M NaCl. The quantification of fucoidan was performed by HPLC with fluorescence detection. The results suggested that the calibration curve for fucoidan concentration was linear dependent in the limits of 0.5-100 mu g/mL. The lower limit of quantitation (LLOQ) was 0.5 mu g/mL and the lower limit of detection (LLOD) was 0.15 mu g/mL.The intra-day and inter-day precision values were less than 13% and the accuracy ranged from 96.83 to 100.03% at 3 different concentrations. The fucoidan stability of rat plasma at different temperatures and time-points was estimated. The extraction efficiencies ranged from 93.33 to 96.53% and the matrix effect ranged from 92.67 to 95.83%. Method selectivity was evaluated as well. We successfully studied the phaimacokinetic of fucoidan in rat plasma after oral by the validated method. Fucoidan was administered to rats intravenously at a dose of 6 mg/kg and orally at a dose of 20 mg/kg. The C-max was 7.33 mu g/mL within 2 h by oral administration. The initial C-max was 75.59 mu g/mL. The bioavailability of fucoidan after oral administration to rats was 8.91%.