标题：Controlled releasing of SDF-1 alpha in chitosan-heparin hydrogel for endometrium injury healing in rat model
作者：Qi Wenbo; Xu Lijian; Zhao Shuangdan; Zheng Jiahua; Tian Yanpeng; Qi Xuejun; Huang Xianghua; Zhang Jingkun
作者机构：[Qi Wenbo; Zhao Shuangdan; Zheng Jiahua; Tian Yanpeng; Qi Xuejun; Huang Xianghua; Zhang Jingkun] Hebei Med Univ, Hosp 2, Dept Obstet & Gynecol, 215 He 更多
通讯作者地址：Huang, XH (corresponding author), Hebei Med Univ, Hosp 2, Dept Obstet & Gynecol, 215 Heping West Rd, Shijiazhuang 050000, Peoples R China.
来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
关键词：Chitosan; Heparin; Controlled releasing hydrogel; Intrauterine adhesion
摘要：Herein we report a facile approach for chitosan-heparin hydrogels with controlled release manner and their applications for intrauterine adhesion. The sol precursor was converted to gel at physiological temperature in 15 min. FTIR, SEM and swelling test were performed to characterize their compositions, morphologies and stability. In vitro releasing profiles was investigated in PBS solutions. Intrauterine injured rat model was established and treated with different methods. The results of H&E staining, Masson trichrome staining, western blots assay, immunohistochemical staining and immunofluorescence staining revealed that endogenous c-kit positive stem cells (HSCs) were recruited to the injury site and promoted the wound recovery. After 7 days' treatment, uterus treated with SDF-1 alpha releasing hydrogel showed no difference with control group on endometrial thickness, glands number and fibrosis level. This work provides a possible method for intrauterine adhesion healing. (C) 2019 Elsevier B.V. All rights reserved.