标题:Wide sugar substrate specificity of galactokinase from Streptococcus pneumoniae TIGR4
作者:Chen, M.;Chen, L.-L.;Zou, Y.;Xue, M.;Liang, M.;Jin, L.;Guan, W.-Y.;Shen, J.;Wang, W.;Wang, L.;Liu, J.;Wang, P.G.
作者机构:[Chen, M] State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda South Road, Jinan, Shandong 250100, China, National Glycoengine 更多
通讯作者:Wang, PG
通讯作者地址:[Wang, PG]Shandong Univ, State Key Lab Microbial Technol, 27 Shanda S Rd, Jinan 250100, Shandong, Peoples R China.
来源:Carbohydrate research
出版年:2011
卷:346
期:15
页码:2421-2425
DOI:10.1016/j.carres.2011.08.014
关键词:Galactokinase;Streptococcus pneumoniae;Substrate specificity
摘要:Galactokinases (GALK) have attracted significant research attention for their potential application in the enzymatic synthesis of unique sugar phosphates. The galactokinase (GalKSpe4) cloned from Streptococcus pneumoniae TIGR4 had a temperature optimum of 45 °C, and a pH optimum of 8.0. The substrate specificity and kinetics studies revealed that GalKSpe4 had moderate activity toward glucose, in contrast with very low or no activity observed in other previously reported GALKs. Most interestingly, GalKSpe4 exhibited activity for GalNAc, which had never been recorded in other GALKs found by now. This is the first time to report that bacterial GALK can recognize GalNAc.
收录类别:EI;SCOPUS;SCIE
WOS核心被引频次:18
Scopus被引频次:18
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-80053932407&doi=10.1016%2fj.carres.2011.08.014&partnerID=40&md5=e9fea4ef289bfbaed35bbf364f1ce220
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