标题:Combined anticalcification treatment of bovine pericardium with decellularization and hyaluronic acid derivative
作者:Zhu,D.;Jin,L.;Wang,X.;Xu,L.;Liu,T.
通讯作者:Liu, T
作者机构:[Zhu, D] School of Chemistry and Pharmaceutical Engineering, Qilu University of Technology, 250353, Jinan, China;[ Jin, L] School of Chemistry and Pha 更多
会议名称:2nd International Conference on Biomedical Engineering and Biotechnology (iCBEB)
会议日期:OCT 11-13, 2013
来源:Bio-medical materials and engineering
出版年:2014
卷:24
期:1
页码:741-749
DOI:10.3233/BME-130862
关键词:anticalcification;decellularization;Glutaraldehyde fixed bovine pericardium;hyaluronic acid derivative
摘要:The objective of this work was to evaluate the effect of decellularization and hyaluronic acid derivative on the improvement of anticalcification of glutaraldehyde fixed bovine pericardium (GFBP) using a rat subcutaneous implantation model A cell extraction process was employed to remove the cells and cellular components from bovine pericardium (BP), leaving a framework of largely insoluble collagen. Then acellular BP was cross-linked by glutaraldehyde solution and treated with hyaluronic acid derivative (HA-ADH) which was obtained by coupling adipic dihydrazide (ADH) on-COOH of hyaluronic acid (HA). The results of in vivo calcification tests showed that the calcium content was decreased dramatically by decellularization alone (from 28.07±18.87 to 2.44±0.55μg Ca/mg dry tissue after 8 weeks\' implantation), and even less concentration was shown by the combination of HA derivative treatment and decellularization (GFaBP-HA group) (0.25±0.08μg Ca/mg dry tissue after 8 weeks\' implantation). In addition, GFaBP-HA group not only presented a lower degree of calcification, but also showed lower ratios of Ca/P molar, which corresponded to amorphous calcium phosphates. The obtained results indicated that GFaBP-HA was a potential candidate for the manufacture of anticalcification bioprostheses.
收录类别:CPCI-S;EI;SCOPUS;SCIE
WOS核心被引频次:2
Scopus被引频次:2
资源类型:会议论文;期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84891106981&doi=10.3233%2fBME-130862&partnerID=40&md5=55f60e186c1bc0239ca5430c1f59bd39
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