标题:Determination of norcantharidin in mouse tissues by liquid chromatography coupled to tandem mass spectrometry and its tissue distribution study
作者:Zhang,R.;Wang,J.;Yuan,G.;Wei,C.;Liu,X.;Wang,B.;Gao,H.;Guo,R.
作者机构:[Zhang, R] Institute of Clinical Pharmacology, Qilu Hospital, Shandong University, 107 West Wenhua Road, 250012 Jinan, China;[ Wang, J] Department of 更多
通讯作者:Guo, R
通讯作者地址:[Guo, R]Shandong Univ, Qilu Hosp, Inst Clin Pharmacol, 107 W Wenhua Rd, Jinan 250012, Shandong, Peoples R China.
来源:Arzneimittel-Forschung: =Drug Research
出版年:2012
卷:62
期:6
页码:290-294
DOI:10.1055/s-0032-1308980
关键词:LC-MS/MS;norcantharidin;tissue distribution
摘要:The purpose of this study is to determine the concentrations of norcantharidin (CAS NO: 5442-12-6) in mouse tissues and investigate its tissue distribution after intragastric administration of disodium norcantharidate solution. A highly sensitive and specific liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed and validated, using ribavirin (CAS NO: 36791-04-5) as the internal standard (IS). Norcantharidin and IS were extracted from 0.3 mL tissue homogenates using protein precipitation with acetone under acid condition. The analyte was separated on a C18 reverse phase column and analyzed by MS/MS in the multiple reaction monitoring (MRM) mode using ESI with positive ionization, m/z 169→123 for norcantharidin and m/z 267→135 for IS. The developed method was validated over a linear range of concentrations 0.01~5 μg·mL 1 in liver, lung, kidney, stomach, small intestine, uterus and testis, 0.005~0.5 μg·mL 1 in heart, spleen and brain, the correlation coefficients (r 2) were between 0.9918 and 0.9976. The tissue distribution study result was as follows: The AUC 0-t of norcantharidin in tissues was in the order as follows: small intestine, stomach, uterus, kidney, testis, liver, lung, spleen, heart, brain.
收录类别:SCOPUS;SCIE
Scopus被引频次:1
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84862857433&doi=10.1055%2fs-0032-1308980&partnerID=40&md5=a82097279eae4d56c20c3bbc50b090fa
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