标题:F-box protein RAE1 regulates the stability of the aluminum-resistance transcription factor STOP1 in Arabidopsis
作者:Zhang, Yang; Zhang, Jie; Guo, Jinliang; Zhou, Fanglin; Singh, Somesh; Xu, Xuan; Xie, Qi; Yang, Zhongbao; Huang, Chao-Feng
作者机构:[Zhang, Yang; Zhang, Jie; Guo, Jinliang; Zhou, Fanglin; Singh, Somesh; Huang, Chao-Feng] Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Ctr Excelle 更多
通讯作者:Huang, CF;Huang, CF
通讯作者地址:[Huang, CF]Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Ctr Excellence Mol Plant Sci, Natl Key Lab Plant Mol Genet, Shanghai 200032, Peoples R Ch 更多
来源:PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
出版年:2019
卷:116
期:1
页码:319-327
DOI:10.1073/pnas.1814426116
关键词:aluminum toxicity; AtALMT1; E3 ligase; low phosphate response;; ubiquitination
摘要:Aluminum (Al) toxicity is a major factor limiting crop production on acid soils, which represent over 30% of the world's arable land. Some plants have evolved mechanisms to detoxify Al. Arabidopsis, for example, secretes malate via the AtALMT1 transporter to chelate and detoxify Al. The C2H2-type transcription factor STOP1 plays a crucial role in Al resistance by inducing the expression of a set of genes, including AtALMT1. Here, we identify and characterize an F-box protein encoding gene regulation of Atalmt1 expression 1 (RAE1) that regulates the level of STOP1. Mutation and overexpression of RAE1 increases or decreases the expression of AtALMT1 and other STOP1-regulated genes, respectively. RAE1 interacts with and promotes the degradation of STOP1 via the ubiquitin-265 proteasome pathway, while Al stress promotes the accumulation of STOP1. We find that STOP1 up-regulates RAE1 expression by directly binding to the RAE1 promoter, thus forming a negative feedback loop between STOP1 and RAE1. Our results demonstrate that RAE1 influences Al resistance through the ubiquitination and degradation of STOP1.
收录类别:SCOPUS;SCIE
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85059443570&doi=10.1073%2fpnas.1814426116&partnerID=40&md5=5997752c8bc66acca5b3cc686e5130b1
TOP