标题:Sonic hedgehog signaling molecules expression in TGF-β1-induced chondrogenic differentiation of rat mesenchymal stem cells in vitro
作者:Shi, Yingchao ;Jia, Ying ;Zu, Shanshan ;Jia, Yanfei ;Zhang, Xueping ;Sun, Haiji ;Ma, Xiaoli
通讯作者:Sun, H
作者机构:[Shi, Yingchao ;Zhang, Xueping ;Sun, Haiji ] College of Life Science, Shandong Normal University, Jinan 250014, China;[Jia, Ying ;Zu, Shanshan ;Jia, Y 更多
会议名称:5th International Symposium on IT in Medicine and Education, ITME 2013
会议日期:July 19, 2013 - July 21, 2013
来源:Lecture Notes in Electrical Engineering
出版年:2014
卷:269 LNEE
页码:755-762
DOI:10.1007/978-94-007-7618-0_73
摘要:Sonic Hedgehog (SHH) is involved in the induction of early artilaginous differentiation of mesenchymal cells in the limb and in the spine. Transforming growth factor beta 1 (TGF-β1) promotes chondrogenic differentiation of bone marrow mesenchymal stem cells (MSCs), while the signaling pathway by which TGF-β1 affects chondrogenic differentiation remains obscure. This study aimed to investigate expression of SHH-signaling molecules SHH and GLI1 during TGF-β1-induced chondrogenic differentiation of rat MSCs in vitro. TGF-β1 promoted chondrogenic differentiation of MSCs at 10 ng/ml from 7 to 21 days, demonstrated by enhancing cartilage markers collagen type II expression during chondrogenic differentiation. Expressions of Shh and Gli1, were tested by RT-PCR and western blot analysis during chondrogenic differentiation. Expressions of Shh and Gli1 were decreased compared to the control on both mRNA and protein level during TGF-β1-induced chondrogenic differentiation of MSCs from 7 to 21 days. Altogether, these data demonstrate that inactivation of SHH-GLI1 pathway during TGF-β1-induced chondrogenic differentiation of MSCs in vitro. These findings provide new data for the mechanistic link between TGF-β1-induced chondrogenic differentiation and Hedgehog signaling pathway. © Springer Science+Business Media Dordrecht 2014.
收录类别:EI
资源类型:会议论文
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