标题：IMPAIRED MITOCHONDRIAL BIOGENESIS IN HIPPOCAMPI OF RATS WITH CHRONIC SEIZURES
作者：Han, Y.; Lin, Y.; Xie, N.; Xue, Y.; Tao, H.; Rui, C.; Xu, J.; Cao, L.; Liu, X.; Jiang, H.; Chi, Z.
作者机构：[Han, Y.; Xie, N.; Xue, Y.; Tao, H.; Rui, C.; Cao, L.; Liu, X.; Chi, Z.] Shandong Univ, Dept Neurol, Qilu Hosp, Jinan 250012, Peoples R China.; [Lin 更多
通讯作者地址：[Chi, Z]Shandong Univ, Dept Neurol, Qilu Hosp, Jinan 250012, Peoples R China.
关键词：chronic seizures; mitochondrial DNA; mitochondrial biogenesis;; peroxisome proliferator-activated receptor gamma coactivator-1 alpha; (PGC-1 alpha); mitochondrial transcription factor A (Tfam)
摘要：Mitochondrial dysfunction has been suggested to be a contributing factor of epilepsy, but the underlying mechanisms are not completely explored. Mitochondrial biogenesis is involved in regulation of mitochondrial content, morphology, and function. In the current study, we show mitochondrial biogenesis severely impaired in hippocampi of rats with chronic seizures induced by pilocarpine, as evidenced by decreased mitochondria! DNA (mtDNA) content and decreased mtDNA-encoded protein level. Furthermore, we show mtDNA transcription and replication reduced in rats with chronic seizures. These defects were independent of downregulation of mitochondrial biogenesis-related factors, such as peroxisome proliferator-activated receptor gamma coactivator-1, nuclear respiratory factor-1, and mitochondrial transcription factor A (Tfam), but depended on reduced Tfam DNA binding activity. The present study suggests novel mechanisms for mitochondrial dysfunction during chronic seizures. (C) 2011 IBRO. Published by Elsevier Ltd. All rights reserved.