标题:Characterization of a gC1qR from the giant freshwater prawn, Macrobrachium rosenbergii
作者:Ye, Ting;Huang, Xin;Wang, Xian-Wei;Shi, Yan-Ru;Hui, Kai-Min;Ren, Qian
作者机构:[Ye, T] College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, China;[ Huang, X] Jiangsu Key Laboratory for Biodiversity and Biotec 更多
通讯作者:Ren, Q
通讯作者地址:[Ren, Q]Nanjing Normal Univ, Coll Life Sci, Jiangsu Key Lab Biodivers & Biotechnol, 1 Wenyuan Rd, Nanjing 210046, Jiangsu, Peoples R China.
来源:Fish & Shellfish Immunology
出版年:2015
卷:43
期:1
页码:200-208
DOI:10.1016/j.fsi.2014.12.030
关键词:MrgC1qR;Innate immunity;Ficolins;Macrobrachium rosenbergii
摘要:gC1qR, as a multicompartmental and a multifunctional protein, plays an important role in innate immunity. In this study, a gC1qR homolog (MrgC1qR) in the giant freshwater prawn, Macrobrachium rosenbergii was identified. MrgC1qR, a 258-amino-acid polypeptide, shares high identities with gC1qR from other species. MrgC1qR gene was expressed in different tissues and was highest expressed in the hepatopancreas. In addition, the MrgC1qR transcript was significantly enhanced after 6 h of white spot syndrome virus (WSSV) infection or post 2 h, 24 h of Vibrio anguillarum challenge compared to appropriate controls. Moreover, recombinant MrgC1qR (rMrgC1qR) had bacterial binding activity, the result also revealed that rMrgC1qR could bind pathogen-associated molecular patterns (PAMPs) such as LPS or PGN, suggesting that MrgC1qRmight function as a pathogen-recognition receptor (PRR). Furthermore, glutathione S-transferase (GST) pull-down assays showed that rMrgC1qR with GST-tag could bind to rMrFicolin1 or rMrFicolin2 with His-tag. Altogether, these results may demonstrate a role for MrgC1qR in innate immunity in the giant freshwater prawns. (C) 2014 Elsevier Ltd. All rights reserved.
收录类别:SCOPUS;SCIE
WOS核心被引频次:6
Scopus被引频次:7
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84920932268&doi=10.1016%2fj.fsi.2014.12.030&partnerID=40&md5=210306da08a6f8e4c5087c81f54836fb
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