标题：Effects of Photodynamic Therapy Using Yellow LED-light with Concomitant Hypocrellin B on Apoptotic Signaling in Keloid Fibroblasts
作者：Hu, Yongqing; Zhang, Chunmin; Li, Shengli; Jiao, Ya; Qi, Tonggang; Wei, Guo; Han, Gangwen
作者机构：[Hu, Yongqing; Zhang, Chunmin; Wei, Guo; Han, Gangwen] Shandong Univ, Dept Dermatol, Hosp 2, Jinan 250033, Shandong, Peoples R China.; [Li, Shengli] 更多
通讯作者地址：[Han, GW]Peking Univ, Dept Dermatol, Int Hosp, Life Pk Rd 1,Life Sci Pk Zhong Guancun, Beijing 102206, Peoples R China.
来源：INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
关键词：keloid fibroblasts; Hypocrellin B; photodynamic therapy; apoptosis
摘要：Keloid is a common and refractory disease characterized by abnormal fibroblast proliferation and excessive deposition of extracellular matrix components. Hypocrellin B (HB) is a natural perylene quinone photosensitizer. In this experiment, we studied the effects of photodynamic therapy (PDT) using yellow light from light-emitting diode (LED) combined with HB on keloid fibroblasts (KFB) in vitro. Our results showed that HB-LED PDT treatment induced significant KFB apoptosis and decreased KFB cell viability. HB-LED PDT treatment lead to significant BAX upregulation and BCL-2 downregulation in KFB cells, which led to elevation of intracellular free Ca2+ and activation of caspase-3. Our data provides preliminary evidence for the potential of HB-LED PDT as a therapeutic strategy for keloid.