标题：Immobilized enzyme reactors in HPLC and its application in inhibitor screening: A review
作者：Si-Meng Fang;Hai-Na Wang;Zhong-Xi Zhao;Wei-Hong Wang
作者机构：[Fang, S.-M] School of Pharmaceutical Sciences, Shandong University, Jinan, China;[ Wang, H.-N] School of Pharmaceutical Sciences, Shandong University 更多
通讯作者地址：[Wang, W.-H] School of Pharmaceutical Sciences, Shandong University, Jinan, China;
关键词：Immobilized enzyme reactors (IMERs);Molecular bio-chromatography;Screening enzyme inhibitors
摘要：This paper sets out to summarize the literatures based on immobilized enzyme biochromatography and its application in inhibitors screening in the last decade.In order to screen enzyme inhibitors from a mass of compounds in preliminary screening,multi-pore materials with good biocompatibility are used for the supports of immobilizing enzymes,and then the immobilized enzyme reactor applied as the inmobilized enzyme stationary phase in HPLC.Therefore,a technology platform of high throughput screening is gradually established to screen the enzyme inhibitors as new anti-tumor drugs.Here,we briefly summarize the selective methods of supports,immobilization techniques,co-immobilized enzymes system and the screening model.