标题:Immobilized enzyme reactors in HPLC and its application in inhibitor screening: A review
作者:Si-Meng Fang;Hai-Na Wang;Zhong-Xi Zhao;Wei-Hong Wang
作者机构:[Fang, S.-M] School of Pharmaceutical Sciences, Shandong University, Jinan, China;[ Wang, H.-N] School of Pharmaceutical Sciences, Shandong University 更多
通讯作者:Wang, WH(wangwh@sdu.edu.cn)
通讯作者地址:[Wang, W.-H] School of Pharmaceutical Sciences, Shandong University, Jinan, China;
来源:药物分析学报(英文)
出版年:2012
卷:2
期:2
页码:83-89
DOI:10.1016/j.jpha.2011.12.002
关键词:Immobilized enzyme reactors (IMERs);Molecular bio-chromatography;Screening enzyme inhibitors
摘要:This paper sets out to summarize the literatures based on immobilized enzyme biochromatography and its application in inhibitors screening in the last decade.In order to screen enzyme inhibitors from a mass of compounds in preliminary screening,multi-pore materials with good biocompatibility are used for the supports of immobilizing enzymes,and then the immobilized enzyme reactor applied as the inmobilized enzyme stationary phase in HPLC.Therefore,a technology platform of high throughput screening is gradually established to screen the enzyme inhibitors as new anti-tumor drugs.Here,we briefly summarize the selective methods of supports,immobilization techniques,co-immobilized enzymes system and the screening model.
收录类别:SCOPUS
Scopus被引频次:12
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84925835418&doi=10.1016%2fj.jpha.2011.12.002&partnerID=40&md5=4d1290b6afaf32f1fa2a97fab97e5abe
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