标题：Fine Mapping of the Maize Cross-Incompatibility Locus Gametophytic Factor 1 (ga1) Using a Homogeneous Population
作者：Liu, Xu; Sun, He; Wu, Ping; Tian, Youhui; Cui, Dezhou; Xu, Chunyan; Li, Song; Li, Peng; Zhang, Hua; Chen, Tingting; Li, Detao; Z 更多 作者机构：[Liu, Xu; Cui, Dezhou; Xu, Chunyan; Li, Song; Li, Peng; Chen, Tingting; Li, Detao] Shandong Agr Univ, State Key Lab Crop Biol, Tai An 271018, Shandong 更多
通讯作者地址：[Xue, YB]Chinese Acad Sci, Inst Genet & Dev Biol, Ctr Dev Biol, Beijing 100101, Peoples R China.
摘要：Phenotyping of a mapping population is usually the bottleneck that limits the size of the mapping population and mapping resolution. A homogeneous population mapping approach was used for mapping maize (Zea mays L.) gametophytic factor 1 (ga1) that could completely eliminate phenotyping during the mapping process. The strong allele of maize ga1 (Ga1-S), from popcorn inbred line SDGa25, showed a 100% cross-incompatibility with the majority of Chinese dent and flint maize. A homogeneous mapping population was developed by crossing an (SDGa25/Jing66) F 1 male back to an SDGa25 female. During the pollination process, pollen grains of ga1 were completely excluded from fertilization, and a homogeneous BC 1 F 1 (SDGa25//SDGa25/Jing66) population was created in which only the Ga1-S/Ga1-S genotype existed, making phenotyping unnecessary. A total of 2245 individuals of this population were genotyped with SD9 and SD12 markers and 20 recombinants were identified. The Ga1-S locus was quickly delineated to a 100 Kb region between markers dCS1 and insertion deletion ID7 at position 9,491,422 and 9,591,946 bp based on the B73 RefGen_v2 sequence. By marker-assisted selection, Ga1-S was introgressed into parental lines of an elite white waxy maize hybrid by six generations of backcrossing and one generation of selfing. The homozygous Ga1-S/Ga1-S hybrid showed full cross-incompatibility to ga1 maize. The Ga1-S allele could be used as a biological reproductive barrier in reducing cross-pollination between different types of maize such as waxy and non-waxy, genetically modified (GM) and non-GM maize.