标题：Effects of mesenchymal stem cells harboring the Interferon-beta gene on A549 lung cancer in nude mice
作者：Chen, Xiao; Wang, Kangwu; Chen, Shijun; Chen, Yuqing
作者机构：[Chen, Xiao; Chen, Yuqing] Bengbu Med Coll, Dept Geratol, Affiliated Hosp 1, Bengbu, Anhui, Peoples R China.; [Chen, Xiao] Shandong Univ, Sch Med, J 更多
通讯作者地址：[Chen, YQ]Shandong Univ, Bengbu Med Coll, Dept Resp Dis, Affiliated Hosp 1,Sch Med, 287 Changhuai Rd, Bengbu 233003, Anhui, Peoples R China.
来源：PATHOLOGY RESEARCH AND PRACTICE
关键词：Interferon-beta; Mesenchymal stem cells; Lung cancer; Tumorigenicity
摘要：Interferon-beta (IFN-beta) exhibits a tumor-killing effect; however, injection of IFN-beta alone for lung cancer is often accompanied by side effects. This study investigated the possibility of using umbilical cord mesenchymal stem cells (MSCs) as cellular carriers of IFN-beta.; Isolated umbilical cord MSCs were transfected with a lentivirus packaging IFN-beta-overexpression plasmid. A549 cells were subcutaneously injected into nude mice to establish a non-small cell lung cancer (NSCLC) mouse model. A total of 50 mice were randomly assigned to 5 different groups: a control group, IFN-beta group, IFN-beta-MSCs group, MSCs-lentivirus group, and MSCs group. Next, the IFN-beta-MSCs, MSCs-lentivirus, and MSCs were injected into the A549 lung cancer-bearing mice in the IFN-beta-MSCs, MSCs-lentivirus and MSCs groups, respectively. Mice in the control and IFN-beta groups were injected with solvent or IFN-beta solution. The tumors in nude mice in the IFN-beta and IFN-beta-MSCs groups grew at significantly slower rates than tumors in the control group, and tumors in the MSCs-lentivirus and MSC groups also grew slowly. The rates of tumor cell apoptosis in the IFN-beta and IFN-beta-MSCs groups were significantly higher than those in the MSCs-lentivirus and MSCs groups. The livers, lungs, and kidneys of nude mice in the IFN-beta group displayed hyperemia, exudation, and pathological lesions, while those of nude mice in the IFN-beta-MSCs group showed no abnormal changes. Both INF-beta-MSCs and INF-beta inhibited the growth of subcutaneously implanted lung tumors; however, INF-beta-MSCs specifically targeted the tumor cells, and did not produce the damage to internal organs caused by the use of INF-beta alone.