标题:RETRACTED: MiR-497 promotes metastasis of colorectal cancer cells through Nrdp1 inhibition (Retracted article. See April, 2017)
作者:Jiang, Yongsheng; Meng, Qinghua; Qi, Jiaqin; Shen, Haiyu; Sun, Shaochuan
作者机构:[Jiang, Yongsheng; Meng, Qinghua; Qi, Jiaqin; Shen, Haiyu; Sun, Shaochuan] Shandong Univ, Dept Gen Surg, Jinan Cent Hosp, Jinan 250013, Peoples R Chin 更多
通讯作者:Sun, SC
通讯作者地址:[Sun, SC]Shandong Univ, Dept Gen Surg, Jinan Cent Hosp, 105 Jiefang Rd, Jinan 250013, Peoples R China.
来源:TUMOR BIOLOGY
出版年:2015
卷:36
期:10
页码:7641-7647
DOI:10.1007/s13277-015-3489-9
关键词:Neuregulin receptor degradation protein-1 (Nrdp1); Colorectal cancer; (CRC); MMP7; miR-497
摘要:We have recently shown that Nrdp1 inhibits phosphorylation of ErB3 in colorectal cancer (CRC) cells, to suppress epidermal growth factor receptor (EGFR) signaling-stimulated MMP7 activation for CRC metastasis. In this study, we examined the control of Nrdp1 in CRC cells. We detected significant increases in miR-497 in CRC specimen, compared to paired normal colorectal tissue. Moreover, we detected a strong positive correlation between miR-497 levels and Nrdp1 levels, and a strong inverse correlation between miR-497 levels and MMP7 levels. In vitro, overexpression of miR-497 in human CRC cells significantly decreased Nrdp1 transcripts and protein, and vice versa. Moreover, overexpression of miR-497 in human CRC cells also significantly increased MMP7 transcripts, cellular protein, and secreted protein, resulting in increases in cell invasiveness in a transwell cell migration assay. Furthermore, we found that MiR-497 directly targeted 3'-UTR of Nrdp1 mRNA to inhibit its translation. Together, our data suggest that the regulation of MMP7 by Nrdp1 in CRC cells could be inhibited by miR-497 through suppressing Nrdp1 translation. Our work thus highlights a novel molecular regulatory machinery that regulates metastasis of CRC.
收录类别:SCOPUS;SCIE
WOS核心被引频次:15
Scopus被引频次:13
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84944278507&doi=10.1007%2fs13277-015-3489-9&partnerID=40&md5=f75f371c6ab49bf98cbc893a061d0ad0
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